ArticleJournal of clinical microbiology1994
Genetic variation in Staphylococcus aureus coagulase genes: potential and limits for use as epidemiological marker.
Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Characterization of methicillin-resistant3 Biotech · 2020Article
- Genetic Diversity and Virulence Factors ofInternational journal of microbiology · 2020Article
- Coagulase gene polymorphism, enterotoxigenecity, biofilm production, and antibiotic resistance in Staphylococcus aureus isolated from bovine raw milk in North West India.Annals of clinical microbiology and antimicrobials · 2017Article
- Molecular typing of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from Shiraz teaching hospitals by PCR-RFLP of coagulase gene.Iranian journal of microbiology · 2014Article
- The Frequency of Methicillin-Resistant Staphylococcus aureus and Coagulase Gene Polymorphism in Egypt.International journal of bacteriology · 2014Article
- Coagulase gene polymorphism of Staphylococcus aureus isolated from clinical and sub-clinical bovine mastitis in Isfahan and Chaharmahal va Bakhtiari provinces of Iran.Comparative clinical pathology · 2011Article
- Usefulness of PCR-restriction fragment length polymorphism typing of the coagulase gene to discriminate arbekacin-resistant methicillin-resistant Staphylococcus aureus strains.Journal of clinical microbiology · 2007Article
- Antimicrobial susceptibility patterns and characterization of clinical isolates of Staphylococcus aureus in KwaZulu-Natal province, South Africa.BMC infectious diseases · 2006Article
- Coagulase gene typing of Staphylococcus aureus isolated from cows with mastitis in southeastern Brazil.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2005Article
- Molecular diagnostics of clinically important staphylococci.Folia microbiologica · 2004Review
- Multiplex PCR protocol for the diagnosis of staphylococcal infection.Journal of clinical microbiology · 2001Article
- Comparison of pulsed-field gel electrophoresis and coagulase gene restriction profile analysis techniques in the molecular typing of Staphylococcus aureus.Journal of clinical microbiology · 2000Article
- Follow-up of Staphylococcus aureus nasal carriage after 8 years: redefining the persistent carrier state.Journal of clinical microbiology · 1999Article
- Validation of binary typing for Staphylococcus aureus strains.Journal of clinical microbiology · 1999Article
- Dissemination of two methicillin-resistant Staphylococcus aureus clones exhibiting negative staphylase reactions in intensive care units.Journal of clinical microbiology · 1999Article
- Evaluation of mannitol salt agar for detection of oxacillin resistance in Staphylococcus aureus by disk diffusion and agar screening.Journal of clinical microbiology · 1998Article
- Short-sequence DNA repeats in prokaryotic genomes.Microbiology and molecular biology reviews : MMBR · 1998Review
- Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene.Journal of clinical microbiology · 1998Article
- Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus.Journal of clinical microbiology · 1998Article
- Typing of Staphylococcus aureus and Staphylococcus epidermidis strains by PCR analysis of inter-IS256 spacer length polymorphisms.Journal of clinical microbiology · 1997Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To perform coagulase gene typing, the repeated units encoding hypervariable regions of the Staphylococcus aureus coagulase gene were amplified by the PCR technique; this was followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP) patterns. In order to assess the discriminatory power of this typing method, 30 epidemiologically unrelated S. aureus strains which differed by their pulsed-field gel electrophoresis patterns were examined. Although 18 of the 30 strains had unique and unshared AluI RFLP patterns, there were only four observed patterns in the remaining 12 strains. This finding indicated that unrelated strains may share identical AluI RFLP patterns. To elucidate the degree of genetic variation in the C-terminus-encoding loci within the coagulase genes, the PCR products of these 12 strains were subjected to Taq polymerase-mediated sequencing. Sequence analysis confirmed the AluI recognition sites in each of the four RFLP groups and demonstrated that AluI appears to yield the highest RFLP in restriction enzyme analysis. By their DNA sequences the majority of strains sharing common AluI groups could be clearly differentiated from each other and revealed between 93.2 and 98.5% homology. When we determined the nucleotide sequences of two strains after six subcultivations no significant alterations were observed. Because the discriminatory power of the current coagulase gene typing method is not great enough to be used as the sole method to type S. aureus, additional techniques are necessary. Sequence analysis of the repeated unit-encoding region for the typing of S. aureus may be potentially useful as an alternative to other current molecular typing techniques.
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