ArticleThe EMBO journal1994
Assembly and disassembly of spliceosomes along a specific pre-messenger RNP fiber.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 65 citations in OpenAlex.
- Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.The Journal of cell biology · 2015Article
- Cotranscriptional splicing efficiency differs dramatically between Drosophila and mouse.RNA (New York, N.Y.) · 2012Article
- Nascent-seq indicates widespread cotranscriptional pre-mRNA splicing in Drosophila.Genes & development · 2011Article
- Total RNA sequencing reveals nascent transcription and widespread co-transcriptional splicing in the human brain.Nature structural & molecular biology · 2011Article
- Real-time imaging of cotranscriptional splicing reveals a kinetic model that reduces noise: implications for alternative splicing regulation.The Journal of cell biology · 2011Article
- Review
- A genome-wide analysis indicates that yeast pre-mRNA splicing is predominantly posttranscriptional.Molecular cell · 2006Article
- A specific SR protein binds preferentially to the secretory protein gene transcripts in salivary glands of Chironomus tentans.Chromosoma · 2006Article
- Functional coupling of RNAP II transcription to spliceosome assembly.Genes & development · 2006Article
- Chironomus tentans-repressor splicing factor represses SR protein function locally on pre-mRNA exons and is displaced at correct splice sites.Molecular biology of the cell · 2006Article
- Conspicuous accumulation of transcription elongation repressor hrp130/CA150 on the intron-rich Balbiani ring 3 gene.Chromosoma · 2004Article
- Cotranscriptional recruitment of the U1 snRNP to intron-containing genes in yeast.Molecular and cellular biology · 2003Article
- An actin-ribonucleoprotein interaction is involved in transcription by RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A novel conserved RNA-binding domain protein, RBD-1, is essential for ribosome biogenesis.Molecular biology of the cell · 2002Article
- The mRNA export factor Dbp5 is associated with Balbiani ring mRNP from gene to cytoplasm.The EMBO journal · 2002Article
- Assembly and transport of a premessenger RNP particle.Proceedings of the National Academy of Sciences of the United States of America · 2001Review
- In situ transcription and splicing in the Balbiani ring 3 gene.The EMBO journal · 2001Article
- Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo.The Journal of cell biology · 1998Article
- The hrp23 protein in the balbiani ring pre-mRNP particles is released just before or at the binding of the particles to the nuclear pore complex.The Journal of cell biology · 1998Article
- Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA.The EMBO journal · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptionally active Balbiani ring (BR) genes in the salivary glands of the dipteran Chironomus tentans were studied by immunoelectron microscopy to establish the distribution of spliceosome components along a specific pre-messenger ribonucleoprotein (pre-mRNP) fiber. The BR genes are 35-40 kb in size with three introns close to the 5' end and one close to the 3' end; a very large middle portion lacks introns. As a rule the 5' introns are spliced concomitant with transcription in the promoter proximal third of the gene, while the 3' intron is spliced post-transcriptionally. The BR genes with growing pre-mRNPs were visualized in situ, while completed and released pre-mRNPs were isolated from the nucleoplasm and studied unfolded on a grid surface. An anti-snRNP antibody (Y12) bound mainly to the promoter proximal third of the BR gene (86%) and only to a minor extent to the middle and distal thirds (7 and 7% respectively). An antibody to an hnRNP protein reacted with the proximal, middle and distal regions to an increasing extent (17, 38 and 45% respectively), reflecting the increase in size of the growing transcription product. In the nucleoplasmic pre-mRNP particle only one end of the RNP fiber was labeled by Y 12, presumably the 3' end; the anti-hnRNP antibody decorated the entire RNP fiber. Thus, the snRNPs do not associate along the whole pre-mRNP fiber but rather bind to the 5' and 3' ends, i.e. the regions containing the introns. The results also imply that the spliceosomes both assemble and disassemble rapidly on the pre-mRNP fiber.
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