ArticleMolecular biology of the cell1994
Multiple N-ethylmaleimide-sensitive components are required for endosomal vesicle fusion.
Article in Molecular biology of the cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 85 citations in OpenAlex.
- A small-molecule competitive inhibitor of phosphatidic acid binding by the AAA+ protein NSF/Sec18 blocks the SNARE-priming stage of vacuole fusion.The Journal of biological chemistry · 2019Article
- P2XThe Journal of physiology · 2018Article
- Role of DHA in aging-related changes in mouse brain synaptic plasma membrane proteome.Neurobiology of aging · 2016Article
- Delivery of nucleic acids and nanomaterials by cell-penetrating peptides: opportunities and challenges.BioMed research international · 2015Review
- Sealing of transected neurites of rat B104 cells requires a diacylglycerol PKC-dependent pathway and a PKA-dependent pathway.Cellular and molecular neurobiology · 2013Article
- Protein-tyrosine phosphatase 1B modulates early endosome fusion and trafficking of Met and epidermal growth factor receptors.The Journal of biological chemistry · 2011Article
- A novel site of action for alpha-SNAP in the SNARE conformational cycle controlling membrane fusion.Molecular biology of the cell · 2008Article
- Distinct roles for the AAA ATPases NSF and p97 in the secretory pathway.Molecular biology of the cell · 2004Article
- Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Release of kinesin from vesicles by hsc70 and regulation of fast axonal transport.Molecular biology of the cell · 2000Article
- Putative fusogenic activity of NSF is restricted to a lipid mixture whose coalescence is also triggered by other factors.The EMBO journal · 2000Article
- Homotypic fusion of immature secretory granules during maturation in a cell-free assay.The Journal of cell biology · 1998Article
- Molecular aspects of the endocytic pathway.The Biochemical journal · 1998Review
- A yeast t-SNARE involved in endocytosis.Molecular biology of the cell · 1998Article
- Formation and turnover of NSF- and SNAP-containing "fusion" complexes occur on undocked, clathrin-coated vesicle-derived membranes.Molecular biology of the cell · 1998Article
- The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function.The EMBO journal · 1998Article
- Fusion of lysosomes with late endosomes produces a hybrid organelle of intermediate density and is NSF dependent.The Journal of cell biology · 1998Article
- Inhibition of endosome fusion by wortmannin persists in the presence of activated Rab5.Molecular biology of the cell · 1998Article
- Stimulation of NSF ATPase activity by alpha-SNAP is required for SNARE complex disassembly and exocytosis.The Journal of cell biology · 1997Article
- Cleavage of rabaptin-5 blocks endosome fusion during apoptosis.The EMBO journal · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This report examines the inhibition of endosomal vesicle fusion by the alkylating agent N-ethylmaleimide (NEM). The concentration of NEM required to inhibit vesicle fusion depended upon whether membrane and cytosolic fractions were treated separately or together, enabling the resolution of at least two components to the inhibition. The first component is inactivated at low levels of NEM when cytosolic and membrane fractions are treated together. On the contrary, inhibition of the second component required higher levels of NEM but was achieved by treating cytosol and membranes separately. Reconstitution studies indicated that both components were cytosolic and that neither corresponded to the ubiquitous NEM-sensitive fusion protein (NSF). The role of NSF in this fusion reaction was further examined using salt-washed membranes depleted of NSF protein. Under these conditions the fusion reaction was fully dependent upon added NSF whose activity, in this context, was sensitive to NEM treatment. From these data we conclude that NSF activity during endosomal vesicle fusion can be dissected into several steps, only a subset of which (perhaps attachment of NSF to the membrane) are sensitive to NEM. Fusion between salt-washed endosomal membranes was also dependent on soluble NSF attachment proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.