ArticlePlant molecular biology1994
A method for examining expression of homologous genes in plant polyploids.
Article in Plant molecular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Evolution and expression of MYB genes in diploid and polyploid cotton.Plant molecular biology · 2003Article
- Genetic and epigenetic interactions in allopolyploid plants.Plant molecular biology · 2000Review
- Construction of Brassica B genome synteny groups based on chromosomes extracted from three different sources by phenotypic, isozyme and molecular markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
- Fruit-specific expression of a defensin-type gene family in bell pepper. Upregulation during ripening and upon wounding.Plant physiology · 1996Article
- Ribulose-1,5-bisphosphate carboxylase/oxygenase gene expression and diversity of Lake Erie planktonic microorganisms.Applied and environmental microbiology · 1996Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the essential issues regarding evolution of polyploid species is how duplicate genes are expressed. Most studies on gene expression in polyploids have been based on isozyme analyses; RNA analysis has not been widely used partially due to difficulties in distinguishing homologous transcripts which usually have the same length and similar or almost identical sequences. In this study, a method combining RT-PCR with RFLP was used to analyze transcripts of homologous genes in natural and synthetic Brassica amphidiploids. Sequences coding for several known genes were selected and used to synthesize gene-specific primers. Total RNAs were used as templates for RT-PCR to amplify homologous transcripts in three diploid parental species, three cultivated amphidiploid species and six synthetic amphidiploids. For each gene, initial PCR products amplified in all species had identical length; however, homologous transcripts in the diploid and amphidiploid species could be distinguished after digesting the PCR products with restriction enzymes. Preliminary results based on three genes indicated that both transcripts from the diploid parents were expressed in the synthetic and natural amphidiploids. This study represents the first application of RT-PCR and RFLP analysis to investigate expression of homologous genes in higher plants. The technique is a sensitive, simple and efficient method for distinguishing homologous transcripts in a mixed RNA population and can be applied to many types of studies on expression of homologous genes.
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