ArticleProceedings of the National Academy of Sciences of the United States of America1994
Identification of calreticulin as a rubella virus RNA binding protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 130 citations in OpenAlex.
- The 3' end of the coding region of senecavirus A contains a highly conserved sequence that potentially forms a stem-loop structure required for virus rescue.Archives of virology · 2023Article
- Calreticulin regulates vascular endothelial growth factor-A mRNA stability in gastric cancer cells.PloS one · 2019Article
- James Neil Gilliam, MD-the career arc of a patient-oriented translational clinical investigation changemaker in rheumatologic skin disease.Annals of translational medicine · 2018Article
- Calreticulin modulates the intracellular survival of mycobacteria by regulating ER-stress-mediated apoptosis.Oncotarget · 2017Article
- Is the Antitumor Property of Trypanosoma cruzi Infection Mediated by Its Calreticulin?Frontiers in immunology · 2016Review
- Hijacking of host calreticulin is required for the white spot syndrome virus replication cycle.Journal of virology · 2014Article
- Is it all That Bad When Living with an Intracellular Protozoan? The Role of Trypanosoma cruzi Calreticulin in Angiogenesis and Tumor Growth.Frontiers in oncology · 2014Review
- Arginylated calreticulin at plasma membrane increases susceptibility of cells to apoptosis.The Journal of biological chemistry · 2012Article
- A physical interaction network of dengue virus and human proteins.Molecular & cellular proteomics : MCP · 2011Article
- Endoplasmic reticulum calcium regulates the retrotranslocation of Trypanosoma cruzi calreticulin to the cytosol.PloS one · 2010Article
- Viral calciomics: interplays between Ca2+ and virus.Cell calcium · 2009Review
- Article
- Post-translational arginylation of calreticulin: a new isospecies of calreticulin component of stress granules.The Journal of biological chemistry · 2007Article
- Retrotranslocation of the chaperone calreticulin from the endoplasmic reticulum lumen to the cytosol.Molecular and cellular biology · 2005Article
- Article
- Analysis of the 3' cis-acting elements of rubella virus by using replicons expressing a puromycin resistance gene.Journal of virology · 2004Article
- Competition of CUGBP1 and calreticulin for the regulation of p21 translation determines cell fate.The EMBO journal · 2004Article
- Article
- Cellular proteins from human monocytes bind to dengue 4 virus minus-strand 3' untranslated region RNA.Journal of virology · 2003Article
- Calreticulin interacts with C/EBPalpha and C/EBPbeta mRNAs and represses translation of C/EBP proteins.Molecular and cellular biology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we observed that sequences at the 3' end of rubella virus (RV) genomic RNA that form a stable stem-loop structure are necessary for initiation of RNA replication. A cytosolic protein found in Vero 76 cells (simian origin) specifically bound to the 3' (+)-stem-loop sequence. In the present study, we have purified the RNA binding protein and identified it as a simian homologue of human calreticulin. The purified calreticulin binds to the RV RNA with specificity similar to the protein present in cytosolic extracts. Human calreticulin antibodies recognize several forms of simian calreticulin, one of which is phosphorylated in vivo. A 2-fold increase in phosphorylation of this form of calreticulin is observed in RV-infected cells. Recombinant human calreticulin can bind RV 3' (+)-stem-loop RNA only after undergoing in vitro phosphorylation. This binding activity is abrogated by pretreatment of phosphorylated recombinant human calreticulin with alkaline phosphatase. The RV RNA was also immunoprecipitated from RV-infected UV-crosslinked Vero 76 cells by using calreticulin antibodies. Our results show that phosphorylated calreticulin is an RNA binding protein and phosphorylation is necessary for this activity. Specific binding of calreticulin to the cis-acting element of RV RNA in vivo suggests a possible role for this interaction in viral replication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.