ArticleProceedings of the National Academy of Sciences of the United States of America1994
A distinct class of intracellular storage vesicles, identified by expression of the glucose transporter GLUT4.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 85 citations in OpenAlex.
- Enhancement of optical force acting on vesicles via the binding of gold nanoparticles.Royal Society open science · 2019Article
- Effect of Three Statins on Glucose Uptake of Cardiomyocytes and its Mechanism.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- The neuroendocrine phenotype of gastric myofibroblasts and its loss with cancer progression.Carcinogenesis · 2014Article
- Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Cerebellar neurons possess a vesicular compartment structurally and functionally similar to Glut4-storage vesicles from peripheral insulin-sensitive tissues.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Translocation of the insulin-regulated aminopeptidase to the cell surface: detection by radioligand binding.British journal of pharmacology · 2008Article
- Glut4 storage vesicles without Glut4: transcriptional regulation of insulin-dependent vesicular traffic.Molecular and cellular biology · 2004Article
- The zinc transporter ZnT3 interacts with AP-3 and it is preferentially targeted to a distinct synaptic vesicle subpopulation.Molecular biology of the cell · 2004Article
- Insulin-responsive compartments containing GLUT4 in 3T3-L1 and CHO cells: regulation by amino acid concentrations.Molecular and cellular biology · 2001Article
- Identification of discrete classes of endosome-derived small vesicles as a major cellular pool for recycling membrane proteins.Molecular biology of the cell · 2001Article
- Characterization of insulin-responsive GLUT4 storage vesicles isolated from 3T3-L1 adipocytes.Molecular and cellular biology · 2000Article
- Differential regulation of secretory compartments containing the insulin-responsive glucose transporter 4 in 3T3-L1 adipocytes.Molecular biology of the cell · 1999Article
- Two compartments for insulin-stimulated exocytosis in 3T3-L1 adipocytes defined by endogenous ACRP30 and GLUT4.The Journal of cell biology · 1999Article
- Doc2 is not associated with known regulated exocytotic or endosomal compartments in adrenal chromaffin cells.The Biochemical journal · 1999Article
- Arrest of endosome acidification by bafilomycin A1 mimics insulin action on GLUT4 translocation in 3T3-L1 adipocytes.The Biochemical journal · 1999Article
- Recombinant major vault protein is targeted to neuritic tips of PC12 cells.The Journal of cell biology · 1999Article
- Depolarization and cAMP elevation rapidly recruit TrkB to the plasma membrane of CNS neurons.Neuron · 1998Article
- Sorting and storage during secretory granule biogenesis: looking backward and looking forward.The Biochemical journal · 1998Review
- GLUT4 and transferrin receptor are differentially sorted along the endocytic pathway in CHO cells.The Journal of cell biology · 1998Article
- Two distinct populations of synaptic-like vesicles from rat brain.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Some cell types have cytoplasmic storage vesicles whose fusion with the cell surface is triggered by an extracellular signal. To explore the relationship between different classes of storage vesicles, we expressed, in the neuro-endocrine cell line PC12, the facilitative glucose transporter GLUT4, which is stored in small cytoplasmic vesicles in fat and muscle cells and mobilized to the cell surface when insulin is present. PC12 cells have two known types of storage vesicles, secretory granules and synaptic vesicles, but GLUT4 is targeted to neither. It is recovered, however, in a class of small vesicles that sediment approximately twice as fast as synaptic vesicles. Immunoelectron microscopy confirmed the presence of such small vesicles in transfected PC12 cells. By velocity sedimentation analysis, GLUT4 vesicles efficiently exclude the synaptic vesicle markers synaptophysin, SV2, and synaptobrevin; the transferrin receptor, a marker of conventional endocytosis; and the polymeric immunoglobulin receptor, a marker of transcytosis. The exclusion of synaptophysin and the transferrin receptor from most of the GLUT4-containing structures was confirmed by confocal immunofluorescence microscopy. Like synaptic vesicles, therefore, GLUT4 vesicles of PC12 cells appear to be a unique type of organelle. A GLUT4-containing organelle of identical sedimentation properties was found in transfected fibroblast cell lines and in rat adipocytes. On stimulation of the adipocytes with insulin, GLUT4 was translocated from the peak of small vesicles to faster sedimenting membranes. We propose that the class of vesicles described here is present in a wide range of cell types and is involved in transient modification of the cell surface.
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