ArticleProceedings of the National Academy of Sciences of the United States of America1994
Identification of synaptic proteins and their isoform mRNAs in compartments of pancreatic endocrine cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 203 citations in OpenAlex.
- Gβγ-SNAP25 exocytotic brake removal enhances insulin action, promotes adipocyte browning, and protects against diet-induced obesity.The Journal of clinical investigation · 2023Article
- Comparative analysis of potential broad-spectrum neuronal Cre drivers.Wellcome open research · 2022Article
- Syntaxin 1: A Novel Robust Immunophenotypic Marker of Neuroendocrine Tumors.International journal of molecular sciences · 2020Article
- Exocytosis proteins as novel targets for diabetes prevention and/or remediation?American journal of physiology. Regulatory, integrative and comparative physiology · 2017Review
- A microRNA negative feedback loop downregulates vesicle transport and inhibits fear memory.eLife · 2016Article
- Characterization of phospholipids in insulin secretory granules and mitochondria in pancreatic beta cells and their changes with glucose stimulation.The Journal of biological chemistry · 2015Article
- Haploinsufficiency of Dmxl2, encoding a synaptic protein, causes infertility associated with a loss of GnRH neurons in mouse.PLoS biology · 2014Article
- Insulin secretion from beta cells in intact mouse islets is targeted towards the vasculature.Diabetologia · 2014Article
- Microarray analysis of Xenopus endoderm expressing Ptf1a.Genesis (New York, N.Y. : 2000) · 2012Article
- Association with nitric oxide synthase on insulin secretory granules regulates glucokinase protein levels.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Pancreatic function, type 2 diabetes, and metabolism in aging.International journal of endocrinology · 2012Article
- Gelsolin associates with the N terminus of syntaxin 4 to regulate insulin granule exocytosis.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Munc18-1 and Munc18-2 proteins modulate beta-cell Ca2+ sensitivity and kinetics of insulin exocytosis differently.The Journal of biological chemistry · 2011Article
- Mechanisms of biphasic insulin-granule exocytosis - roles of the cytoskeleton, small GTPases and SNARE proteins.Journal of cell science · 2009Review
- The role of the t-SNARE SNAP-25 in action potential-dependent calcium signaling and expression in GABAergic and glutamatergic neurons.BMC neuroscience · 2008Article
- The identification of potential factors associated with the development of type 2 diabetes: a quantitative proteomics approach.Molecular & cellular proteomics : MCP · 2008Article
- Imaging analysis reveals mechanistic differences between first- and second-phase insulin exocytosis.The Journal of cell biology · 2007Article
- Initiation of male sperm-transfer behavior in Caenorhabditis elegans requires input from the ventral nerve cord.BMC biology · 2006Article
- Neuronal calcium sensor-1 potentiates glucose-dependent exocytosis in pancreatic beta cells through activation of phosphatidylinositol 4-kinase beta.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Correlation of syntaxin-1 and SNAP-25 clusters with docking and fusion of insulin granules analysed by total internal reflection fluorescence microscopy.Diabetologia · 2004Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several proteins that are of importance for membrane trafficking in the nerve terminal have recently been characterized. We have used Western blot and immunohistochemistry to show that synaptotagmin, synaptobrevin/VAMP (vesicle-associated membrane protein), SNAP-25 (synaptosomal-associated protein of 25 kDa), and syntaxin proteins are present in cells of the islets of Langerhans in the endocrine pancreas. Synaptotagmin-like immunoreactivity (-LI) was localized to granules within the cytoplasm of a few endocrine cells located in the periphery of the islets, identified as somatostatin-containing cells, and in many nerve fibers within the islets. VAMP-LI was seen in granules of virtually all pancreatic islet cells and also in nerve fibers. SNAP-25-LI and syntaxin-LI were predominantly present in the plasma membrane of the endocrine cells, including insulin-producing beta cells. In situ hybridization, using isoform-specific oligonucleotide probes, detected VAMP-2, cellubrevin, SNAP-25, syntaxin 1A, 4, and 5, and munc-18 mRNAs in isolated pancreatic islets and in insulin-producing cells. The results show the presence of several synaptic proteins at protein and mRNA levels in pancreatic islet cells, suggesting that they may have specific roles in the molecular regulation of exocytosis also in insulin-secreting cells.
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