ArticleThe Journal of cell biology1994
Expression of a myosin regulatory light chain phosphorylation site mutant complements the cytokinesis and developmental defects of Dictyostelium RMLC null cells.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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35 citing papers in PubMed, 93 citations in OpenAlex.
- Article
- Particle-based model of mechanosensory contractility kit assembly.Biophysical journal · 2022Article
- Filament evanescence of myosin II and smooth muscle function.The Journal of general physiology · 2021Review
- Various Themes of Myosin Regulation.Journal of molecular biology · 2016Review
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- Nonmuscle myosin-2: mix and match.Cellular and molecular life sciences : CMLS · 2013Review
- Cytokinesis mechanics and mechanosensing.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Cytokinesis through biochemical-mechanical feedback loops.Seminars in cell & developmental biology · 2010Review
- Cytotoxic Effects on HL-60 Cells of Myosin Light Chain Kinase Inhibitor ML-7 Alone and in Combination with Flavonoids.Toxicological research · 2009Article
- Regulation of fission yeast myosin-II function and contractile ring dynamics by regulatory light-chain and heavy-chain phosphorylation.Molecular biology of the cell · 2009Article
- Biological, biochemical, and kinetic effects of mutations of the cardiomyopathy loop of Dictyostelium myosin II: importance of ALA400.The Journal of biological chemistry · 2005Article
- Mechanics and regulation of cytokinesis.Current opinion in cell biology · 2004Review
- Formation of Hirano bodies induced by expression of an actin cross-linking protein with a gain-of-function mutation.Eukaryotic cell · 2003Article
- Quantitation of the distribution and flux of myosin-II during cytokinesis.BMC cell biology · 2002Article
- Functional elements within the dynein microtubule-binding domain.Molecular biology of the cell · 2000Article
- Parameters that specify the timing of cytokinesis.The Journal of cell biology · 1999Article
- Activation of myosin phosphatase targeting subunit by mitosis-specific phosphorylation.The Journal of cell biology · 1999Article
- Filament structure as an essential factor for regulation of Dictyostelium myosin by regulatory light chain phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- MLCK-A, an unconventional myosin light chain kinase from Dictyostelium, is activated by a cGMP-dependent pathway.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Specific localization of serine 19 phosphorylated myosin II during cell locomotion and mitosis of cultured cells.The Journal of cell biology · 1998Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
In a number of systems phosphorylation of the regulatory light chain (RMLC) of myosin regulates the activity of myosin. In smooth muscle and vertebrate nonmuscle systems RMLC phosphorylation is required for contractile activity. In Dictyostelium discoideum phosphorylation of the RMLC regulates both ATPase activity and motor function. We have determined the site of phosphorylation on the Dictyostelium RMLC and used site-directed mutagenesis to replace the phosphorylated serine with an alanine. The mutant light chain was then expressed in RMLC null Dictyostelium cells (mLCR-) from an actin promoter on an integrating vector. The mutant RMLC was expressed at high levels and associated with the myosin heavy chain. RMLC bearing a ser13ala substitution was not phosphorylated in vitro by purified myosin light chain kinase, nor could phosphate be detected on the mutant RMLC in vivo. The mutant myosin had reduced actin-activated ATPase activity, comparable to fully dephosphorylated myosin. Unexpectedly, expression of the mutant RMLC rescued the primary phenotypic defects of the mlcR- cells to the same extent as did expression of wild-type RMLC. These results suggest that while phosphorylation of the Dictyostelium RMLC appears to be tightly regulated in vivo, it is not essential for myosin-dependent cellular functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.