ArticleMolecular and cellular biology1995
Calspermin gene transcription is regulated by two cyclic AMP response elements contained in an alternative promoter in the calmodulin kinase IV gene.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 112 citations in OpenAlex.
- Characterization of the promoter region of the murine Catsper2 gene.FEBS open bio · 2022Article
- Experimental induction and mathematical modeling ofChannels (Austin, Tex.) · 2020Article
- Common variants in genes of the postsynaptic FMRP signalling pathway are risk factors for autism spectrum disorders.Human genetics · 2014Article
- Ca2+-signaling, alternative splicing and endoplasmic reticulum stress responses.Neurochemical research · 2011Review
- Hormone-sensitive lipase expression and IHC localization in the rat ovary, oviduct, and uterus.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Human gastrin-releasing peptide receptor gene regulation requires transcription factor binding at two distinct CRE sites.American journal of physiology. Gastrointestinal and liver physiology · 2008Article
- cAMP-response element modulator-tau activates a distinct promoter element for the expression of the phospholipid hydroperoxide/sperm nucleus glutathione peroxidase gene.The Biochemical journal · 2004Article
- Review
- Spermatogenesis and the regulation of Ca(2+)-calmodulin-dependent protein kinase IV localization are not dependent on calspermin.Molecular and cellular biology · 2001Article
- The CREM system in human spermatogenesis.Journal of endocrinological investigation · 2000Review
- Activation of orphan receptor-mediated transcription by Ca(2+)/calmodulin-dependent protein kinase IV.The EMBO journal · 2000Article
- Regulated expression of focal adhesion kinase-related nonkinase, the autonomously expressed C-terminal domain of focal adhesion kinase.Molecular and cellular biology · 1999Article
- A novel 14-base-pair regulatory element is essential for in vivo expression of murine beta4-galactosyltransferase-I in late pachytene spermatocytes and round spermatids.Molecular and cellular biology · 1999Article
- The role of autosomal cell apoptosis regulator genes in human spermatogenesis.International urology and nephrology · 1999Article
- The mouse ADP-ribosylation factor-like 4 gene: two separate promoters direct specific transcription in tissues and testicular germ cell.The Biochemical journal · 1998Article
- cAMP-response element modulator tau is a positive regulator of testis angiotensin converting enzyme transcription.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- An alternative, nonkinase product of the brain-specifically expressed Ca2+/calmodulin-dependent kinase II alpha isoform gene in skeletal muscle.Molecular and cellular biology · 1996Article
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
Abstract
The transcript for the high-affinity Ca2+/calmodulin-binding protein calspermin is generated from the gene encoding Ca2+/calmodulin-dependent protein kinase IV only in postmeiotic germ cells during spermatogenesis. We demonstrate that this testis-specific calspermin transcript can be produced in heterologous cells by utilization of a promoter located in an intron of the calmodulin (CaM) kinase IV gene. Critical motifs within this promoter are two cyclic AMP response element (CRE)-like sequences located about -70 and -50 bp upstream of the transcriptional initiation site. Both CRE motifs are footprinted by the authentic testis-specific transcriptional activator CREM tau or by CREM tau present in adult testis nuclear extract. Whereas a 2.1-kb DNA fragment containing the calspermin promoter is inactive when transfected into NIH 3T3 cells, activity can be restored by cotransfection of CREM tau and protein kinase A or CaM kinase IV but not CaM kinase II alpha. Restoration of activity is greatly reduced by mutation of the two CRE motifs. Since CRE-like motifs have been identified in many genes uniquely expressed in postmeiotic germ cells, which contain abundant CREM tau protein, we suggest that CREM tau may function as one transcription factor responsible for the expression of postmeiotic germ cell-specific genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.