ArticleMolecular and cellular biology1995
Long-range RNA interaction of two sequence elements required for endonucleolytic cleavage of human insulin-like growth factor II mRNAs.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
- The complex genetics of human insulin-like growth factor 2 are not reflected in public databases.The Journal of biological chemistry · 2018Article
- Proteomic analysis of Chinese hamster ovary cells.Journal of proteome research · 2012Article
- Review
- Control of fetal growth and neonatal survival by the RasGAP-associated endoribonuclease G3BP.Molecular and cellular biology · 2005Article
- Preliminary physical mapping of RNA-RNA linkages in the genomic RNA of Moloney murine leukemia virus.Journal of virology · 2005Article
- RNA destabilization by the granulocyte colony-stimulating factor stem-loop destabilizing element involves a single stem-loop that promotes deadenylation.Molecular and cellular biology · 2002Article
- Comparative sequence analysis of the imprinted Dlk1-Gtl2 locus in three mammalian species reveals highly conserved genomic elements and refines comparison with the Igf2-H19 region.Genome research · 2001Article
- The fragile X mental retardation protein binds specifically to its mRNA via a purine quartet motif.The EMBO journal · 2001Article
- Kinetics and regulation of site-specific endonucleolytic cleavage of human IGF-II mRNAs.Nucleic acids research · 2001Article
- Structural and functional analysis of an mRNP complex that mediates the high stability of human beta-globin mRNA.Molecular and cellular biology · 2001Article
- Identification of RNA sequences and structures involved in site-specific cleavage of IGF-II mRNAs.RNA (New York, N.Y.) · 1998Article
- mRNA instability elements in the human papillomavirus type 16 L2 coding region.Journal of virology · 1998Article
- Posttranscriptional regulation of collagen alpha1(I) mRNA in hepatic stellate cells.Molecular and cellular biology · 1997Article
- A mouse cytoplasmic exoribonuclease (mXRN1p) with preference for G4 tetraplex substrates.The Journal of cell biology · 1997Article
- Cleavage properties of an estrogen-regulated polysomal ribonuclease involved in the destabilization of albumin mRNA.Nucleic acids research · 1997Article
- Growth-condition-dependent regulation of insulin-like growth factor II mRNA stability.The Biochemical journal · 1996Article
- The cis-acting elements involved in endonucleolytic cleavage of the 3' UTR of human IGF-II mRNAs bind a 50 kDa protein.Nucleic acids research · 1996Article
- mRNA stability in mammalian cells.Microbiological reviews · 1995Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human insulin-like growth factor II (IGF-II) mRNAs are subject to site-specific endonucleolytic cleavage in the 3' untranslated region, leading to an unstable 5' cleavage product containing the IGF-II coding region and a very stable 3' cleavage product of 1.8 kb. This endonucleolytic cleavage is most probably the first and rate-limiting step in degradation of IGF-II mRNAs. Two sequence elements within the 3' untranslated region are required for cleavage: element I, located approximately 2 kb upstream of the cleavage site, and element II, encompassing the cleavage site itself. We have identified a stable double-stranded RNA stem structure (delta G = -100 kcal/mol [418.4 kJ/mol]) that can be formed between element I and a region downstream of the cleavage site in element II. This structure is conserved among human, rat, and mouse mRNAs. Detailed analysis of the requirements for cleavage shows that the relative position of the elements is not essential for cleavage. Furthermore, the distance between the coding region and the cleavage site does not affect the cleavage reaction. Mutational analysis of the long-range RNA-RNA interaction shows that not only the double-stranded character but also the sequence of the stable RNA stem is important for cleavage.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.