ArticleMolecular and cellular biology1995
Position-independent transgene expression mediated by boundary elements from the apolipoprotein B chromatin domain.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 127 citations in OpenAlex.
- Review
- Identification of a potent MAR element from the human genome and assessment of its activity in stably transfected CHO cells.Journal of cellular and molecular medicine · 2018Article
- High-level transgene expression by homologous recombination-mediated gene transfer.Nucleic acids research · 2011Article
- Development of a BAC vector for integration-independent and tight regulation of transgenes in rodents via the Tet system.Transgenic research · 2011Article
- In vivo expression of MHC class I genes depends on the presence of a downstream barrier element.PloS one · 2009Article
- A nuclear matrix attachment site in the 4q35 locus has an enhancer-blocking activity in vivo: implications for the facio-scapulo-humeral dystrophy.Genome research · 2008Article
- Identification, genome mapping, and CTCF binding of potential insulators within the FXYD5-COX7A1 locus of human chromosome 19q13.12.Mammalian genome : official journal of the International Mammalian Genome Society · 2006Article
- Human matrix attachment regions are necessary for the establishment but not the maintenance of transgene insulation in Drosophila melanogaster.Molecular and cellular biology · 2004Article
- The c-myc insulator element and matrix attachment regions define the c-myc chromosomal domain.Molecular and cellular biology · 2003Article
- Functional dissection of the mouse tyrosinase locus control region identifies a new putative boundary activity.Nucleic acids research · 2003Article
- Identification of an insulator in AAVS1, a preferred region for integration of adeno-associated virus DNA.Journal of virology · 2003Article
- Locus control regions.Blood · 2002Review
- Evaluation of sequence motifs found in scaffold/matrix-attached regions (S/MARs).Nucleic acids research · 2002Article
- Small tumor virus genomes are integrated near nuclear matrix attachment regions in transformed cells.Journal of virology · 2001Article
- Stable expression and cell-specific chromatin structure of human alpha1-antitrypsin cosmid transgenes in rat hepatoma cells.Nucleic acids research · 2000Article
- Nuclear matrix attachment regions antagonize methylation-dependent repression of long-range enhancer-promoter interactions.Genes & development · 1999Article
- The lawc gene is a new member of the trithorax-group that affects the function of the gypsy insulator of Drosophila.Genetics · 1999Article
- Insulation of a conditionally expressed transgene in an adenoviral vector.Gene therapy · 1999Article
- Dividing the empire: boundary chromatin elements delimit the territory of enhancers.The EMBO journal · 1999Review
- Forum domain in Drosophila melanogaster cut locus possesses looped domains inside.Nucleic acids research · 1998Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
The human apolipoprotein B (apoB) gene resides within a 47.5-kb chromatin domain that is flanked by sequences that bind to the nuclear matrix. These matrix attachment regions (MARs) are boundaries between nuclease-sensitive and -resistant chromatin. As domain boundaries are thought to function as insulator elements, shielding sequences between them from effects of neighboring chromatin, this raised the possibility that the apoB MARs have functions that could be assayed by transfection. To test this possibility, we examined effects of the apoB MARs on transgene expression in transiently and stably transfected rat and human hepatoma cells. The apoB MARs had no effects on expression of transiently transfected reporters, but they altered expression of stably integrated transgenes in dramatic and reproducible ways. Single integrated copies of transgenes that contained the apoB promoter and second intron enhancer, which are sufficient for high-level expression in transient assays, were expressed at low and variable levels in stable transfectant clones. In contrast, transgenes containing the apoB 5' and 3' MARs were expressed at levels nearly 200-fold higher than levels of the minimal reporters in stable transfectants, and expression was position independent. Transgenes that contained the apoB MARs and an additional 3.3 kb of apoB 5' flanking sequence were also expressed in an elevated, position-independent manner. Surprisingly, tandem transgene arrays in multicopy transfectants were transcriptionally inactive. These observations suggest that the apoB MARs function as insulator elements, shielding transgene expression from effects of neighboring chromatin domains.
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