ArticleThe Journal of cell biology1994
Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 114 citations in OpenAlex.
- Intrinsically disordered regions facilitate Mlp1-Nab2 recognition in mRNA quality control.Nucleus (Austin, Tex.) · 2026Article
- TPR is a prognostic biomarker and potential therapeutic target associated with immune infiltration in hepatocellular carcinoma.Molecular and clinical oncology · 2024Article
- Distinct roles of nuclear basket proteins in directing the passage of mRNA through the nuclear pore.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Mechanosensing at the Nuclear Envelope by Nuclear Pore Complex Stretch Activation and Its Effect in Physiology and Pathology.Frontiers in physiology · 2019Article
- AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint.Cell cycle (Georgetown, Tex.) · 2017Article
- RegPhos 2.0: an updated resource to explore protein kinase-substrate phosphorylation networks in mammals.Database : the journal of biological databases and curation · 2014Article
- Challenges in homology search: HMMER3 and convergent evolution of coiled-coil regions.Nucleic acids research · 2013Article
- Regulation of autophagy by nucleoporin Tpr.Scientific reports · 2012Article
- Studies of phosphoproteomic changes induced by nucleophosmin-anaplastic lymphoma kinase (ALK) highlight deregulation of tumor necrosis factor (TNF)/Fas/TNF-related apoptosis-induced ligand signaling pathway in ALK-positive anaplastic large cell lymphoma.Molecular & cellular proteomics : MCP · 2010Article
- The nuclear pore complex: bridging nuclear transport and gene regulation.Nature reviews. Molecular cell biology · 2010Review
- Nuclear transport and the mitotic apparatus: an evolving relationship.Cellular and molecular life sciences : CMLS · 2010Review
- Nucleoporin translocated promoter region (Tpr) associates with dynein complex, preventing chromosome lagging formation during mitosis.The Journal of biological chemistry · 2010Article
- Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM.Cell cycle (Georgetown, Tex.) · 2010Article
- Double duty for nuclear proteins--the price of more open forms of mitosis.Trends in genetics : TIG · 2009Review
- Evidence for a shared nuclear pore complex architecture that is conserved from the last common eukaryotic ancestor.Molecular & cellular proteomics : MCP · 2009Article
- Nuclear accumulation of polyglutamine disease proteins and neuropathology.Molecular brain · 2009Review
- Nuclear pore proteins and cancer.Seminars in cell & developmental biology · 2009Review
- Functional significance of the interaction between the mRNA-binding protein, Nab2, and the nuclear pore-associated protein, Mlp1, in mRNA export.The Journal of biological chemistry · 2008Article
- Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket.Molecular biology of the cell · 2004Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
From a panel of monoclonal antibodies raised against fractions of rat liver nuclear envelopes (NEs), we have identified an antibody, RL30, which reacts with novel nuclear pore complex (NPC) antigens that are not O-glycosylated. By immunofluorescence staining of cultured cells, RL30 reacts exclusively with the NE in a punctate pattern that largely coincides with that of identified NPC proteins. RL30 labels only the cytoplasmic surface of the NPC in immunogold electron microscopy, predominantly in peripheral regions nearby the cytoplasmic ring. In immunoblots of isolated rat liver NEs and cultured rat cells, RL30 recognizes a 265-kD band, as well as a series of 175-265-kD bands in rat liver NEs that are likely to be proteolytic products of p265. Sequencing of peptides from the 175- and 265-kD RL30 antigens of rat liver revealed that they are both closely related to human Tpr, a protein whose amino-terminal 150-250 amino acids appear in oncogenic fusions with the kinase domains of the met, trk, and raf protooncogenes. We found that in vitro translation of human Tpr mRNA yields a major 265-kD band. Considered together, these data indicate that the 265-kD RL30 antigen in the NPC is the rat homologue of Tpr. Interestingly, Tpr contains an exceptionally long predicted coiled coil domain (approximately 1600 amino acids). The localization and predicted structure of Tpr suggest that it is a component of the cytoplasmic fibrils of the NPC implicated in nuclear protein import. Immunofluorescence microscopy shows that during NPC reassembly at the end of mitosis, Tpr becomes concentrated at the NE significantly later than O-linked glycoproteins, including p62. This indicates that reassembly of the NPC after mitosis is a stepwise process, and that the Tpr-containing peripheral structures are assembled later than p62.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.