ArticleApplied and environmental microbiology1995
Increased expression of Aspergillus parasiticus aflR, encoding a sequence-specific DNA-binding protein, relieves nitrate inhibition of aflatoxin biosynthesis.
Article in Applied and environmental microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 208 citations in OpenAlex.
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- Advanced mycotoxin control and decontamination techniques in view of an increased aflatoxin risk in Europe due to climate change.Frontiers in microbiology · 2022Review
- Endophytic Fungi: From Symbiosis to Secondary Metabolite Communications or Vice Versa?Frontiers in plant science · 2021Review
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- The Methyltransferase AflSet1 Is Involved in Fungal Morphogenesis, AFB1 Biosynthesis, and Virulence ofFrontiers in microbiology · 2020Article
- Resilience of Biocontrol for Aflatoxin Minimization Strategies: Climate Change Abiotic Factors May Affect Control in Non-GM and GM-Maize Cultivars.Frontiers in microbiology · 2019Article
- Biocontrol ofFrontiers in microbiology · 2018Article
- Expression of ustR and the Golgi protease KexB are required for ustiloxin B biosynthesis in Aspergillus oryzae.AMB Express · 2016Article
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- A Network Approach of Gene Co-expression in theFrontiers in genetics · 2016Article
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- Examining the evolution of the regulatory circuit controlling secondary metabolism and development in the fungal genus Aspergillus.PLoS genetics · 2015Article
- Variability of chromosome structure in pathogenic fungi--of 'ends and odds'.Current opinion in microbiology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aflR gene from Aspergillus parasiticus and Aspergillus flavus may be involved in the regulation of aflatoxin biosynthesis. The aflR gene product, AFLR, possesses a GAL4-type binuclear zinc finger DNA-binding domain. A transformant, SU1-N3 (pHSP), containing an additional copy of aflR, showed increased transcription of aflR and the aflatoxin pathway structural genes, nor-1, ver-1, and omt-1, when cells were grown in nitrate medium, which normally suppresses aflatoxin production. Electrophoretic mobility shift assays showed that the recombinant protein containing the DNA-binding domain, AFLR1, bound specifically to the palindromic sequence, TTAGGCCTAA, 120 bp upstream of the AFLR translation start site. Expression of aflR thus appears to be autoregulated. Increased expression of aflatoxin biosynthetic genes in the transformant might result from an elevated basal level of AFLR, allowing it to overcome nitrate inhibition and to bind to the aflR promotor region, thereby initiating aflatoxin biosynthesis. Results further suggest that aflR is involved in the regulation of multiple parts of the aflatoxin biosynthetic pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.