ArticleApplied and environmental microbiology1995
Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei beta-mannanase gene containing a cellulose binding domain.
Article in Applied and environmental microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 179 citations in OpenAlex.
- Review
- LncRNA regulates tomato fruit cracking by coordinating gene expression via a hormone-redox-cell wall network.BMC plant biology · 2020Article
- Transcriptome analysis of the salivary glands of the grain aphid, Sitobion avenae.Scientific reports · 2017Article
- A new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris.BMC biotechnology · 2014Article
- Comparison of expression systems for the extracellular production of mannanase Man23 originated from Bacillus subtilis B23.Microbial cell factories · 2013Article
- Article
- Re-annotation of the CAZy genes of Trichoderma reesei and transcription in the presence of lignocellulosic substrates.Microbial cell factories · 2012Article
- Expression of lignocellulolytic enzymes in Pichia pastoris.Microbial cell factories · 2012Article
- Podospora anserina hemicellulases potentiate the Trichoderma reesei secretome for saccharification of lignocellulosic biomass.Applied and environmental microbiology · 2011Article
- Expression of Trichoderma reesei β-mannanase in tobacco chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis.PloS one · 2011Article
- Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-beta-mannosidase from Bacillus licheniformis in Escherichia coli.Microbial cell factories · 2010Article
- Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01.Microbial cell factories · 2009Article
- Heterologous expression and optimized production of an Aspergillus aculeatus endo-1,4-beta-mannanase in Yarrowia lipolytica.Molecular biotechnology · 2009Article
- Production of the Aspergillus aculeatus endo-1,4-beta-mannanase in A. niger.Journal of industrial microbiology & biotechnology · 2009Article
- Novel Coprinopsis cinerea polyesterase that hydrolyzes cutin and suberin.Applied and environmental microbiology · 2009Article
- Increased production of xylanase by expression of a truncated version of the xyn11A gene from Nonomuraea flexuosa in Trichoderma reesei.Applied and environmental microbiology · 2007Article
- Carbohydrate binding modules: biochemical properties and novel applications.Microbiology and molecular biology reviews : MMBR · 2006Review
- A mannanase, ManA, of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 has carbohydrate binding and docking modules.Canadian journal of microbiology · 2005Article
- Role of the bga1-encoded extracellular {beta}-galactosidase of Hypocrea jecorina in cellulase induction by lactose.Applied and environmental microbiology · 2005Article
- The ire1 and ptc2 genes involved in the unfolded protein response pathway in the filamentous fungus Trichoderma reesei.Molecular genetics and genomics : MGG · 2004Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
beta-Mannanase (endo-1,4-beta-mannanase; mannan endo-1,4-beta-mannosidase; EC 3.2.1.78) catalyzes endo-wise hydrolysis of the backbone of mannan and heteromannans, including hemicellulose polysaccharides, which are among the major components of plant cell walls. The gene man1, which encodes beta-mannanase, of the filamentous fungus Trichoderma reesei was isolated from an expression library by using antiserum raised towards the earlier-purified beta-mannanase protein. The deduced beta-mannanase consists of 410 amino acids. On the basis of hydrophobic cluster analysis, the beta-mannanase was assigned to family 5 of glycosyl hydrolases (cellulase family A). The C terminus of the beta-mannanase has strong amino acid sequence similarity to the cellulose binding domains of fungal cellulases and is preceded by a serine-, threonine-, and proline-rich region. Consequently, the beta-mannanase is probably organized similarly to the T. reesei cellulases, having a catalytic core domain separated from the substrate-binding domain by an O-glycosylated linker. Active beta-mannanase was expressed and secreted by using the yeast Saccharomyces cerevisiae as the host. The results indicate that the man1 gene encodes the two beta-mannanases with different isoelectric points (pIs 4.6 and 5.4) purified earlier from T. reesei.
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