ArticleMolecular and cellular biology1995
Functional mRNA can be generated by RNA polymerase III.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 65 citations in OpenAlex.
- R-loops orchestrate RNAPII transcriptional reprogramming for the maternal-to-zygotic transition.Cell research · 2026Article
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- Yeast U6 snRNA made by RNA polymerase II is less stable but functional.RNA (New York, N.Y.) · 2022Article
- Retroviral Antisense Transcripts and Genes: 33 Years after First Predicted, a Silent Retroviral Revolution?Viruses · 2021Review
- Lytic Infection with Murine Gammaherpesvirus 68 Activates Host and Viral RNA Polymerase III Promoters and Enhances Noncoding RNA Expression.Journal of virology · 2021Article
- Further analysis of barley MORC1 using a highly efficient RNA-guided Cas9 gene-editing system.Plant biotechnology journal · 2018Article
- RNA Polymerase II Activity of Type 3 Pol III Promoters.Molecular therapy. Nucleic acids · 2018Article
- A researcher's guide to the galaxy of IRESs.Cellular and molecular life sciences : CMLS · 2017Review
- Mutation of nucleotides around the +1 position of type 3 polymerase III promoters: The effect on transcriptional activity and start site usage.Transcription · 2017Article
- RNA polymerase III accurately initiates transcription from RNA polymerase II promoters in vitro.The Journal of biological chemistry · 2014Article
- The mammalian orthoreovirus bicistronic M3 mRNA initiates translation using a 5' end-dependent, scanning mechanism that does not require interaction of 5'-3' untranslated regions.Virus research · 2014Article
- A common strategy for host RNA degradation by divergent viruses.Journal of virology · 2012Article
- Mechanisms of the initiation of protein synthesis: in reading frame binding of ribosomes to mRNA.Molecular biology reports · 2011Article
- A dual interface determines the recognition of RNA polymerase II by RNA capping enzyme.The Journal of biological chemistry · 2010Article
- Transcription termination in the plasmid/virus hybrid pSSVx from Sulfolobus islandicus.Extremophiles : life under extreme conditions · 2010Article
- Persistent growth of a human plasma-derived hepatitis C virus genotype 1b isolate in cell culture.PLoS pathogens · 2010Article
- Transcripts synthesized by RNA polymerase III can be polyadenylated in an AAUAAA-dependent manner.RNA (New York, N.Y.) · 2008Article
- A polymerase III-like reinitiation mechanism is operating in regulation of histone expression in archaea.Molecular microbiology · 2008Article
- Type and level of RMRP functional impairment predicts phenotype in the cartilage hair hypoplasia-anauxetic dysplasia spectrum.American journal of human genetics · 2007Article
- Human Maf1 negatively regulates RNA polymerase III transcription via the TFIIB family members Brf1 and Brf2.International journal of biological sciences · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Eukaryotic cellular mRNA is believed to be synthesized exclusively by RNA polymerase II (pol II), whereas pol I produces long rRNAs and pol III produces 5S rRNA, tRNA, and other small RNAs. To determine whether this functional differentiation is obligatory, we examined the translational potential of an artificial pol III transcript. The coding region of the human immunodeficiency virus type 1 tat gene was placed under the control of a strong pol III promoter from the adenovirus type 2 VA RNAI gene. The resultant chimera, pVA-Tat, was transcribed accurately in vivo and in vitro and gave rise to Tat protein, which transactivated a human immunodeficiency virus-driven chloramphenicol acetyltransferase reporter construct in transfected HeLa cells. pol III-specific mutations down-regulated VA-Tat RNA production in vivo and in vitro and dramatically reduced chloramphenicol acetyltransferase transactivation. As expected for a pol III transcript, VA-Tat RNA was not detectably capped at its 5' end or polyadenylated at its 3' end, but, like mRNA, it was associated with polysomes in a salt-stable manner. Mutational analysis of a short open reading frame upstream of the Tat-coding sequence implicates scanning in the initiation of VA-Tat RNA translation despite the absence of a cap. In comparison with tat mRNA generated by pol II, VA-Tat RNA was present on smaller polysomes and was apparently translated less efficiently, which is consistent with a relatively low initiation rate. Evidently, human cells are capable of utilizing pol III transcripts as functional mRNAs, and neither a cap nor a poly(A) tail is essential for translation, although they may be stimulatory. These findings raise the possibility that some cellular mRNAs are made by pol I or pol III.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.