ArticleMolecular and cellular biology1995
Differential roles of two tandem E2F sites in repression of the human p107 promoter by retinoblastoma and p107 proteins.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 112 citations in OpenAlex.
- The Tumor Suppressor p53 Downregulates p107 (International journal of molecular sciences · 2025Article
- Cell Death in Chondrocytes, Osteoblasts, and Osteocytes.International journal of molecular sciences · 2016Review
- Characterization of the 5'-flanking region of the human TP53 gene and its response to the natural compound, Resveratrol.Journal of biochemistry · 2016Article
- The association of mammalian DREAM complex and HPV16 E7 proteins.American journal of cancer research · 2015Review
- PP2A: more than a reset switch to activate pRB proteins during the cell cycle and in response to signaling cues.Cell cycle (Georgetown, Tex.) · 2015Review
- Regulation of Rb family proteins by Cdk6/Ccnd1 in growth plates.Cell cycle (Georgetown, Tex.) · 2013Article
- Article
- p16(INK4a) -mediated suppression of telomerase in normal and malignant human breast cells.Aging cell · 2010Article
- Tandem E2F binding sites in the promoter of the p107 cell cycle regulator control p107 expression and its cellular functions.PLoS genetics · 2010Article
- p107 in the public eye: an Rb understudy and more.Cell division · 2010Article
- Regulation of RB transcription in vivo by RB family members.Molecular and cellular biology · 2010Article
- Review
- GFP reporter mice for the retinoblastoma-related cell cycle regulator p107.Cell cycle (Georgetown, Tex.) · 2008Article
- Article
- A distance difference matrix approach to identifying transcription factors that regulate differential gene expression.Genome biology · 2007Article
- Visualizing dynamic E2F-mediated repression in vivo.Molecular and cellular biology · 2006Article
- Primary and compensatory roles for RB family members at cell cycle gene promoters that are deacetylated and downregulated in doxorubicin-induced senescence of breast cancer cells.Molecular and cellular biology · 2006Article
- Down-regulation of the retinoblastoma tumor suppressor by the hepatitis C virus NS5B RNA-dependent RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Two E2F elements regulate the proliferating cell nuclear antigen promoter differently during leaf development.The Plant cell · 2002Article
- B-Myb overcomes a p107-mediated cell proliferation block by interacting with an N-terminal domain of p107.Oncogene · 2002Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Although many lines of evidence indicate that the cellular protein p107 is closely related to the retinoblastoma protein, the exact function of the p107 gene and its regulation are presently not known. To investigate the molecular mechanism controlling expression of the human p107 gene, a 5' flanking sequence of this gene was isolated and shown to promote high-level expression of a luciferase reporter gene in cycling human 293 and Saos-2 cells. Sequencing and transcription mapping analyses showed that the human p107 promoter is TATA-less and contains a tandem, direct repeat of E2F-binding sites, with the 3' copy overlapping the major transcription initiation site. Deletion analysis of the p107 promoter showed that a promoter DNA fragment containing only the two E2F sites together with the leader sequence could direct relatively efficient expression in 293 cells. Site-directed mutagenesis of these E2F sites revealed that although both sites were important for p107 promoter activity, mutation on the proximal, initiation site copy of the E2F site showed a stronger effect. The human p107 promoter could be repressed by the retinoblastoma protein and its own gene product. Interestingly, the repression was found to be mediated through the 5' copy of the E2F site. These studies demonstrate for the first time differential roles of two tandem E2F sites in promoter regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.