ArticleThe Journal of cell biology1995
Transport of dendritic microtubules establishes their nonuniform polarity orientation.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 70 citations in OpenAlex.
- Polarity reversal of stable microtubules during neuronal development.Journal of cell science · 2025Article
- Mechanical Regulation of Neurite Polarization and Growth: A Computational Study.Biophysical journal · 2020Article
- Microtubules: A Key to Understand and Correct Neuronal Defects in CDKL5 Deficiency Disorder?International journal of molecular sciences · 2019Review
- Mitotic Motor KIFC1 Is an Organizer of Microtubules in the Axon.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- The Role of the Microtubule Cytoskeleton in Neurodevelopmental Disorders.Frontiers in cellular neuroscience · 2018Review
- Non-centrosomal nucleation mediated by augmin organizes microtubules in post-mitotic neurons and controls axonal microtubule polarity.Nature communications · 2016Article
- Loss of γ-tubulin, GCP-WD/NEDD1 and CDK5RAP2 from the Centrosome of Neurons in Developing Mouse Cerebral and Cerebellar Cortex.Acta histochemica et cytochemica · 2015Article
- Γ-tubulin controls neuronal microtubule polarity independently of Golgi outposts.Molecular biology of the cell · 2014Article
- Mitotic motors coregulate microtubule patterns in axons and dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Microtubules are organized independently of the centrosome in Drosophila neurons.Neural development · 2011Article
- Hooks and comets: The story of microtubule polarity orientation in the neuron.Developmental neurobiology · 2011Review
- The role of the cytoskeleton in the life cycle of viruses and intracellular bacteria: tracks, motors, and polymerization machines.Current drug targets. Infectious disorders · 2002Article
- Depletion of a microtubule-associated motor protein induces the loss of dendritic identity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- An essential role for katanin in severing microtubules in the neuron.The Journal of cell biology · 1999Article
- Expression of the mitotic motor protein Eg5 in postmitotic neurons: implications for neuronal development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Identification of a microtubule-associated motor protein essential for dendritic differentiation.The Journal of cell biology · 1997Article
- Microtubule organization and stability in the oligodendrocyte.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Inhibition of a mitotic motor compromises the formation of dendrite-like processes from neuroblastoma cells.The Journal of cell biology · 1997Article
- Microtubule assembly in growing dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Tau binds to the distal axon early in development of polarity in a microtubule- and microfilament-dependent manner.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The immature processes that give rise to both axons and dendrites contain microtubules (MTs) that are uniformly oriented with their plus-ends distal to the cell body, and this pattern is preserved in the developing axon. In contrast, developing dendrites gradually acquire nonuniform MT polarity orientation due to the addition of a subpopulation of oppositely oriented MTs (Baas, P. W., M. M. Black, and G. A. Banker. 1989. J. Cell Biol. 109:3085-3094). In theory, these minus-end-distal MTs could be locally nucleated and assembled within the dendrite itself, or could be transported into the dendrite after their nucleation within the cell body. To distinguish between these possibilities, we exposed cultured hippocampal neurons to nanomolar levels of vinblastine after one of the immature processes had developed into the axon but before the others had become dendrites. At these levels, vinblastine acts as a kinetic stabilizer of MTs, inhibiting further assembly while not substantially depolymerizing existing MTs. This treatment did not abolish dendritic differentiation, which occurred in timely fashion over the next two to three days. The resulting dendrites were flatter and shorter than controls, but were identifiable by their ultrastructure, chemical composition, and thickened tapering morphology. The growth of these dendrites was accompanied by a diminution of MTs from the cell body, indicating a net transfer of MTs from one compartment into the other. During this time, minus-end-distal microtubules arose in the experimental dendrites, indicating that new MT assembly is not required for the acquisition of nonuniform MT polarity orientation in the dendrite. Minus-end-distal microtubules predominated in the more proximal region of experimental dendrites, indicating that most of the MTs at this stage of development are transported into the dendrite with their minus-ends leading. These observations indicate that transport of MTs from the cell body is an essential feature of dendritic development, and that this transport establishes the nonuniform polarity orientation of MTs in the dendrite.
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