ArticleThe Journal of cell biology1995
Chemoattractant and GTP gamma S-mediated stimulation of adenylyl cyclase in Dictyostelium requires translocation of CRAC to membranes.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 69 citations in OpenAlex.
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- An endogenous chemorepellent directs cell movement by inhibiting pseudopods at one side of cells.Molecular biology of the cell · 2019Article
- Chemoattractant stimulation of TORC2 is regulated by receptor/G protein-targeted inhibitory mechanisms that function upstream and independently of an essential GEF/Ras activation pathway in Dictyostelium.Molecular biology of the cell · 2013Article
- Signaling mechanisms for chemotaxis.Development, growth & differentiation · 2011Review
- Ras-mediated activation of the TORC2-PKB pathway is critical for chemotaxis.The Journal of cell biology · 2010Article
- Activated cAMP receptors switch encystation into sporulation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A protein with similarity to PTEN regulates aggregation territory size by decreasing cyclic AMP pulse size during Dictyostelium discoideum development.Eukaryotic cell · 2008Article
- Signal relay during chemotaxis.Journal of microscopy · 2008Review
- Activation of Galphai3 triggers cell migration via regulation of GIV.The Journal of cell biology · 2008Article
- A chemoattractant-mediated Gi-coupled pathway activates adenylyl cyclase in human neutrophils.Molecular biology of the cell · 2007Article
- Big roles for small GTPases in the control of directed cell movement.The Biochemical journal · 2007Review
- Cellular asymmetry and individuality in directional sensing.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Phosphoinositide 3-kinase activity controls the chemoattractant-mediated activation and adaptation of adenylyl cyclase.Molecular biology of the cell · 2006Article
- TOR complex 2 integrates cell movement during chemotaxis and signal relay in Dictyostelium.Molecular biology of the cell · 2005Article
- A cell number counting factor regulates Akt/protein kinase B to regulate Dictyostelium discoideum group size.Eukaryotic cell · 2004Article
- Receptor-mediated regulation of PI3Ks confines PI(3,4,5)P3 to the leading edge of chemotaxing cells.Molecular biology of the cell · 2003Article
- Role of phosphatidylinositol 3' kinase and a downstream pleckstrin homology domain-containing protein in controlling chemotaxis in dictyostelium.The Journal of cell biology · 2001Article
- A novel Ras-interacting protein required for chemotaxis and cyclic adenosine monophosphate signal relay in Dictyostelium.Molecular biology of the cell · 1999Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We have previously reported that activation of adenylyl cyclase by chemoattractant receptors in Dictyostelium requires, in addition to a heterotrimeric G-protein, a cytosolic protein, designated CRAC (Lilly, P., and P. N. Devreotes. 1994. J. Biol. Chem. 269:14123-14129; Insall, R. H., A. Kuspa, P. J. Lilly, G. Schaulsky, L. R. Levin, W. F. Loomis, and P. N. Devreotes. 1994. J. Cell Biol. 126:1537-1545). In this report, we show that in intact cells, chemoattractants promote translocation of CRAC from the cytosolic to the membrane fraction. However, CRAC is not required at the time of receptor stimulation; it can be added to lysates of activated cells. Treatment of membranes with guanine nucleotides creates binding sites for CRAC. These binding sites can be generated in mutants lacking each of the components of the pathway except the beta-subunit, suggesting that free or "activated" beta gamma-subunits may be a part of the binding site. This hypothesis is consistent with previous observations that CRAC contains a pleckstrin homology domain and that the beta gamma-subunits likely mediate activation of adenylyl cyclase in this system. Thus, CRAC may serve as an adapter, linking the G-protein beta gamma-subunits to activation of the enzyme. GTP gamma S cannot generate CRAC-binding sites when the adenylyl cyclase pathway has been adapted by prior chemoattractant stimulation, suggesting that this is a point of downstream adaptation.
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