ArticleThe Journal of cell biology1995
Actin polymerization and intracellular solvent flow in cell surface blebbing.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed, 269 citations in OpenAlex.
- Towards a systematic understanding of the diverse subtypes, biogenesis and functions of large extracellular vesicles.Nature reviews. Molecular cell biology · 2026Review
- Influence of the glycocalyx on the size and mechanical properties of plasma membrane-derived vesicles.Soft matter · 2025Article
- Cell blebbing novel therapeutic possibilities to counter metastasis.Clinical & experimental metastasis · 2024Review
- Blebology: principles of bleb-based migration.Trends in cell biology · 2024Review
- Proteolysis-free amoeboid migration of melanoma cells through crowded environments via bleb-driven worrying.Developmental cell · 2024Article
- Model of inverse bleb growth explains giant vacuole dynamics during cell mechanoadaptation.PNAS nexus · 2023Article
- Growth cone macropinocytosis of neurotrophin receptor and neuritogenesis are regulated by neuron navigator 1.Molecular biology of the cell · 2022Article
- Ferroptosis induces membrane blebbing in placental trophoblasts.Journal of cell science · 2022Article
- Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism.Nature communications · 2022Article
- Blebs-Formation, Regulation, Positioning, and Role in Amoeboid Cell Migration.Frontiers in cell and developmental biology · 2022Review
- A role for myosin II clusters and membrane energy in cortex rupture for Dictyostelium discoideum.PloS one · 2022Article
- Physics and Physiology of Cell Spreading in Two and Three Dimensions.Physiology (Bethesda, Md.) · 2021Article
- Dynamic interplay between cell membrane tension and clathrin-mediated endocytosis.Biology of the cell · 2021Review
- Precise Tuning of Cortical Contractility Regulates Cell Shape during Cytokinesis.Cell reports · 2020Article
- Cellular Blebs and Membrane Invaginations Are Coupled through Membrane Tension Buffering.Biophysical journal · 2019Article
- TGFβ-induced cytoskeletal remodeling mediates elevation of cell stiffness and invasiveness in NSCLC.Scientific reports · 2019Article
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Time-resolved ultrastructure of the cortical actin cytoskeleton in dynamic membrane blebs.The Journal of cell biology · 2019Article
- Myosin II-interacting guanine nucleotide exchange factor promotes bleb retraction via stimulating cortex reassembly at the bleb membrane.Molecular biology of the cell · 2018Article
- A combined experimental and theoretical investigation on cellular blebbing.Scientific reports · 2017Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
The cortical actin gel of eukaryotic cells is postulated to control cell surface activity. One type of protrusion that may offer clues to this regulation are the spherical aneurysms of the surface membrane known as blebs. Blebs occur normally in cells during spreading and alternate with other protrusions, such as ruffles, suggesting similar protrusive machinery is involved. We recently reported that human melanoma cell lines deficient in the actin filament cross-linking protein, ABP-280, show prolonged blebbing, thus allowing close study of blebs and their dynamics. Blebs expand at different rates of volume increase that directly predict the final size achieved by each bleb. These rates decrease as the F-actin concentration of the cells increase over time after plating on a surface, but do so at lower concentrations in ABP-280 expressing cells. Fluorescently labeled actin and phalloidin injections of blebbing cells indicate that a polymerized actin structure is not present initially, but appears later and is responsible for stopping further bleb expansion. Therefore, it is postulated that blebs occur when the fluid-driven expansion of the cell membrane is sufficiently rapid to initially outpace the local rate of actin polymerization. In this model, the rate of intracellular solvent flow driving this expansion decreases as cortical gelation is achieved, whether by factors such as ABP-280, or by concentrated actin polymers alone, thereby leading to decreased size and occurrence of blebs. Since the forces driving bleb extension would always be present in a cell, this process may influence other cell protrusions as well.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.