ArticleGene expression1995
Overexpression of the E2F-1 transcription factor gene mediates cell transformation.
Article in Gene expression, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 41 citations in OpenAlex.
- Genistein targets the cancerous inhibitor of PP2A to induce growth inhibition and apoptosis in breast cancer cells.International journal of oncology · 2016Article
- The balance between cell cycle arrest and cell proliferation: control by the extracellular matrix and by contact inhibition.Interface focus · 2014Article
- Enhanced cancer cell killing by truncated E2F-1 used in combination with oncolytic adenovirus.Virology · 2012Article
- E2F-1 lacking the transcriptional activity domain induces autophagy.Cancer biology & therapy · 2012Article
- Construction of a computable cell proliferation network focused on non-diseased lung cells.BMC systems biology · 2011Article
- E2F-1 binding affinity for pRb is not the only determinant of the E2F-1 activity.International journal of biological sciences · 2010Article
- E2F-1 has dual roles depending on the cell cycle.International journal of biological sciences · 2010Article
- Overexpression of SERTAD3, a putative oncogene located within the 19q13 amplicon, induces E2F activity and promotes tumor growth.Oncogene · 2007Article
- A novel targeted therapy of Leydig and granulosa cell tumors through the luteinizing hormone receptor using a hecate-chorionic gonadotropin beta conjugate in transgenic mice.Neoplasia (New York, N.Y.) · 2005Article
- E2F-1 induces proliferation-specific genes and suppresses squamous differentiation-specific genes in human epidermal keratinocytes.Oncogene · 2000Article
- A subset of cells expressing SV40 large T antigen contain elevated p53 levels and have an altered cell cycle phenotype.Cell proliferation · 2000Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The E2F transcription factor can regulate expression of numerous cellular genes controlling proliferation, including proto-oncogenes and genes regulating cell cycle progression. Therefore, genes comprising the E2F gene family could potentially contribute to carcinogenesis. To test the potential of E2F to act as a transforming gene, a cDNA encoding E2F-1 was constitutively overexpressed in established rodent cells using a retroviral vector. Overexpressed E2F-1 was functional, as shown by stimulation of a transfected adenovirus E2 promoter driving a chloramphenicol acetyltransferase reporter gene in E2F-1 overexpressing cells. This stimulation was dependent on functional E2F binding sites in the promoter. Examination of phenotype showed that E2F-1 overexpression mediated cell transformation as measured by the ability of cells to form colonies in soft agar medium. In addition, overexpressed E2F-1 shortened the duration of the G1 cell cycle phase in proliferating cells, a property characteristic of other transforming genes. These data provide direct evidence that E2F-1 can act as a transforming gene and a critical regulator of cell cycle progression and suggest the possibility of E2F involvement in carcinogenesis.
Indexed as
Identifiers
7787412PMC6134389W2462387289What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.