ArticlePlant molecular biology1995
Expression of the CMS-associated urfS sequence in transgenic petunia and tobacco.
Article in Plant molecular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
- Molecular genetics of cytoplasmic male sterility and restorer-of-fertility for the fine tuning of pollen production in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers.Frontiers in plant science · 2015Article
- Mitochondrial cytochrome c oxidase and F1Fo-ATPase dysfunction in peppers (Capsicum annuum L.) with cytoplasmic male sterility and its association with orf507 and Ψatp6-2 genes.International journal of molecular sciences · 2013Article
- Mitochondrially-targeted expression of a cytoplasmic male sterility-associated orf220 gene causes male sterility in Brassica juncea.BMC plant biology · 2010Article
- Article
- Genome barriers between nuclei and mitochondria exemplified by cytoplasmic male sterility.Plant & cell physiology · 2008Review
- Isolation and characterization of the cytoplasmic male sterility-associated orf456 gene of chili pepper (Capsicum annuum L.).Plant molecular biology · 2007Article
- Interactions of mitochondrial and nuclear genes that affect male gametophyte development.The Plant cell · 2004Review
- Aerobic fermentation during tobacco pollen development.Plant molecular biology · 1997Article
- A cytoplasmic male sterility-associated mitochondrial protein causes pollen disruption in transgenic tobacco.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- How do alterations in plant mitochondrial genomes disrupt pollen development?Journal of bioenergetics and biomembranes · 1995Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA which encodes the 25 kDa protein. The urfS sequence was fused with two different mitochondrial targeting sequences. The chimeric gene coding region was placed under the control of the CaMV 35S promoter or a tapetum-specific promoter. Expression of the PCF protein was obtained in mitochondria of transgenic petunia and tobacco plants, yet fertility of the plants was not affected. Analysis of the location of the urfS-encoded protein revealed that it fractionates primarily into the soluble fraction in the transgenic plants whereas the genuine 25 kDa protein is found primarily in the soluble fraction but also in the membrane portion of immature buds from CMS petunia plants. Fertile transgenic plants were obtained which expressed the 25 kDa protein in the tapetal layer of post-meiotic anthers, while CMS plants express the endogenous 25 kDa protein in both the tapetal layer and sporogenous tissue of pre-meiotic anthers.
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