ArticleBiophysical journal1995
Function of the N terminus of the myosin essential light chain of vertebrate striated muscle.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
31 citing papers in PubMed, 69 citations in OpenAlex.
- Myosin essential light chain 1sa decelerates actin and thin filament gliding on β-myosin molecules.The Journal of general physiology · 2022Article
- Cardiac ventricular myosin and slow skeletal myosin exhibit dissimilar chemomechanical properties despite bearing the same myosin heavy chain isoform.The Journal of biological chemistry · 2022Article
- Insights into myosin regulatory and essential light chains: a focus on their roles in cardiac and skeletal muscle function, development and disease.Journal of muscle research and cell motility · 2020Article
- High-throughput screen, using time-resolved FRET, yields actin-binding compounds that modulate actin-myosin structure and function.The Journal of biological chemistry · 2018Article
- Thick Filament Protein Network, Functions, and Disease Association.Comprehensive Physiology · 2018Review
- A Cardiomyopathy Mutation in the Myosin Essential Light Chain Alters Actomyosin Structure.Biophysical journal · 2017Article
- Distinct sequences and post-translational modifications in cardiac atrial and ventricular myosin light chains revealed by top-down mass spectrometry.Journal of molecular and cellular cardiology · 2017Article
- A Novel Method of Determining the Functional Effects of a Minor Genetic Modification of a Protein.Frontiers in cardiovascular medicine · 2015Article
- Mutations of the Drosophila myosin regulatory light chain affect courtship song and reduce reproductive success.PloS one · 2014Article
- Myosin regulatory light chain (RLC) phosphorylation change as a modulator of cardiac muscle contraction in disease.The Journal of biological chemistry · 2013Article
- Characterizations of myosin essential light chain's N-terminal truncation mutant Δ43 in transgenic mouse papillary muscles by using tension transients in response to sinusoidal length alterations.Journal of muscle research and cell motility · 2013Article
- The structural dynamics of actin during active interaction with myosin depends on the isoform of the essential light chain.Biochemistry · 2013Article
- Deletion of 1-43 amino acids in cardiac myosin essential light chain blunts length dependency of Ca(2+) sensitivity and cross-bridge detachment kinetics.American journal of physiology. Heart and circulatory physiology · 2013Article
- Response of rigor cross-bridges to stretch detected by fluorescence lifetime imaging microscopy of myosin essential light chain in skeletal muscle fibers.The Journal of biological chemistry · 2011Article
- Functional differences between the N-terminal domains of mouse and human myosin binding protein-C.Journal of biomedicine & biotechnology · 2010Article
- Species-specific differences in the Pro-Ala rich region of cardiac myosin binding protein-C.Journal of muscle research and cell motility · 2009Review
- Phosphorylation and the N-terminal extension of the regulatory light chain help orient and align the myosin heads in Drosophila flight muscle.Journal of structural biology · 2009Article
- The role of the N-terminus of the myosin essential light chain in cardiac muscle contraction.Journal of molecular biology · 2009Article
- Orientation of the essential light chain region of myosin in relaxed, active, and rigor muscle.Biophysical journal · 2008Article
- GFP-tagged regulatory light chain monitors single myosin lever-arm orientation in a muscle fiber.Biophysical journal · 2007Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
All but one (LC3-f; a fast skeletal muscle isoform) of the essential light chain isoforms of myosin (ELC) that are expressed in vertebrate striated muscles have an extended N terminus that is found neither in invertebrate ELCs nor in the majority of vertebrate smooth and nonmuscle myosin ELCs. Studies with permeabilized skeletal muscle fibers and in vitro motility assays have demonstrated that the presence of the ELC isoform lacking the N-terminal extension (LC3-f) is correlated with an increased maximal velocity of filament sliding. To examine further this modulatory role of the ELCs, a procedure was developed for the exchange of ELCs that is based on a technique for the removal of regulatory light chains from permeabilized muscle fibers. Different isoforms of the ELCs and mutant ELCs were exchanged into permeabilized skeletal muscle fibers from rabbit psoas muscle. The role of the ELCs of myosin in altering the shortening Vmax of striated muscle was confirmed. Additionally, experiments with mutant ELCs in which lysines at the extreme N terminus were replaced with alanines, demonstrated an increased shortening Vmax that coincided with removal of the positive charges contributed by the lysines. This suggests that charge interactions (i.e., salt bridges) between the N terminus of the ELC and negatively charged amino acids on the surface of actin cause a slowing of filament sliding. Whether this role in altering shortening velocity is the primary function of the extended N terminus of the ELC or whether it is merely a consequence of providing a tether between the thick and thin filaments is discussed.
Indexed as
Identifiers
7787052PMC1281889W1735876679What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.