ArticlePlant physiology1995
Purification, characterization, and cDNA cloning of profilin from Phaseolus vulgaris.
Article in Plant physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 54 citations in OpenAlex.
- Profilin and Its Isoforms: Unravelling the Functional Diversity Across Life Forms.Current protein & peptide science · 2026Review
- Legume Allergens Pea, Chickpea, Lentil, Lupine and Beyond.Current allergy and asthma reports · 2024Review
- Proteomics analyses of subcutaneous adipocytes reveal novel abnormalities in human insulin resistance.Obesity (Silver Spring, Md.) · 2016Article
- Domestication-driven Gossypium profilin 1 (GhPRF1) gene transduces early flowering phenotype in tobacco by spatial alteration of apical/floral-meristem related gene expression.BMC plant biology · 2016Article
- Profilin as a regulator of the membrane-actin cytoskeleton interface in plant cells.Frontiers in plant science · 2013Review
- Characterization of PROFILIN genes from allotetraploid (Gossypium hirsutum) cotton and its diploid progenitors and expression analysis in cotton genotypes differing in fiber characteristics.Molecular biology reports · 2012Article
- Article
- Distinct roles of the first introns on the expression of Arabidopsis profilin gene family members.Plant physiology · 2006Article
- Profilin plays a role in cell elongation, cell shape maintenance, and flowering in Arabidopsis.Plant physiology · 2000Article
- Actin-bundling protein isolated from pollen tubes of lily. Biochemical and immunocytochemical characterizationPlant physiology · 1998Article
- Rearrangement of actin microfilaments in plant root hairs responding to rhizobium etli nodulation signalsPlant physiology · 1998Article
- The Arabidopsis profilin gene family. Evidence for an ancient split between constitutive and pollen-specific profilin genes.Plant physiology · 1996Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Profilin from common bean (Phaseolus vulgaris L.) was purified to homogeneity by poly-L-Pro affinity chromatography and gel filtration. The hypocotyl and symbiotic root nodule protein was detected as a single isoform with a 14.4-kD molecular mass and an isoelectric point of 5.3. Partial amino acid and DNA sequencing of a full-length cDNA clone confirmed its identity as profilin. An antibody generated against the purified protein binds to a protein with the same molecular mass in leaves and nodules. Immunolocalization of the protein showed a diffuse distribution in the cytoplasm of hypocotyls and nodules but enhanced staining at the vascular bundles. The strong identity of the sequence among the profilins of birch, maize, and bean suggests that it may play an important role in the signal transduction mechanism of plant cells and plant-bacterial symbioses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.