ArticleNucleic acids research1995
Transcription activation of yeast ribosomal protein genes requires additional elements apart from binding sites for Abf1p or Rap1p.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- The homeostatic regulation of ribosome biogenesis.Seminars in cell & developmental biology · 2023Review
- Regulation of ribosomal protein genes: An ordered anarchy.Wiley interdisciplinary reviews. RNA · 2021Review
- Correspondence: Reply to 'DNA shape is insufficient to explain binding'.Nature communications · 2017Article
- Promoter architecture and transcriptional regulation of Abf1-dependent ribosomal protein genes in Saccharomyces cerevisiae.Nucleic acids research · 2016Article
- Introns regulate the production of ribosomal proteins by modulating splicing of duplicated ribosomal protein genes.Nucleic acids research · 2016Article
- Exocytosis and Endocytosis of Small Vesicles across the Plasma Membrane in Saccharomyces cerevisiae.Membranes · 2014Review
- Two distinct promoter architectures centered on dynamic nucleosomes control ribosomal protein gene transcription.Genes & development · 2014Article
- Article
- Comparative proteomic analysis of transition of saccharomyces cerevisiae from glucose-deficient medium to glucose-rich medium.Proteome science · 2012Article
- Compensation for differences in gene copy number among yeast ribosomal proteins is encoded within their promoters.Genome research · 2011Article
- Hmo1 directs pre-initiation complex assembly to an appropriate site on its target gene promoters by masking a nucleosome-free region.Nucleic acids research · 2011Article
- Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation.The EMBO journal · 2011Article
- Many sequence-specific chromatin modifying protein-binding motifs show strong positional preferences for potential regulatory regions in the Saccharomyces cerevisiae genome.Nucleic acids research · 2010Article
- Fine-structure analysis of ribosomal protein gene transcription.Molecular and cellular biology · 2006Article
- An HMG protein, Hmo1, associates with promoters of many ribosomal protein genes and throughout the rRNA gene locus in Saccharomyces cerevisiae.Molecular and cellular biology · 2006Article
- The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription.Nucleic acids research · 2005Article
- Comparison of ABF1 and RAP1 in chromatin opening and transactivator potentiation in the budding yeast Saccharomyces cerevisiae.Molecular and cellular biology · 2004Article
- Rap1p and other transcriptional regulators can function in defining distinct domains of gene expression.Nucleic acids research · 2003Article
- Osmotic stress signaling and osmoadaptation in yeasts.Microbiology and molecular biology reviews : MMBR · 2002Review
- Different roles for abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene.Nucleic acids research · 2000Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
All ribosomal protein (rp) gene promoters from Saccharomyces cerevisiae studied so far contain either (usually two) binding sites for the global gene regulator Rap1p or one binding site for another global factor, Abf1p. Previous analysis of the rpS33 and rpL45 gene promoters suggested that apart from the Abf1 binding site, additional cis-acting elements play a part in transcription activation of these genes. We designed a promoter reconstruction system based on the beta-glucuronidase reporter gene to examine the role of the Abf1 binding site and other putative cis-acting elements in promoting transcription. An isolated Abf1 binding site turned out to be a weak activating element. A T-rich sequence derived from the rpS33 proximal promoter was found to be stronger, but full transcription activation was only achieved by a combination of these elements. Both in the natural rpL45 promoter and in the reconstituted promoter, a Rap1 binding site could functionally replace the Abf1 binding site. Characteristic rp gene nutritional control of transcription, evoked by a carbon source upshift or by nitrogen re-feeding to nitrogen starved cells, could only be mediated by the combined Abf1 (or Rap1) binding site and T-rich element and not by the individual elements. These results demonstrate that Abf1p and Rap1p do not activate rp genes in a prototypical fashion, but rather may serve to potentiate transcription activation through the T-rich element.
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