ArticleNucleic acids research1995
Site-specific initiation of DNA replication within the non-transcribed spacer of Physarum rDNA.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Telomere-to-telomere characterization of rDNA chromosome in the myxomycete Didymium iridis.BMC molecular and cell biology · 2026Article
- Ribosomal DNA replication time coordinates completion of genome replication and anaphase in yeast.Cell reports · 2023Article
- Article
- A revisionist replicon model for higher eukaryotic genomes.Journal of cellular biochemistry · 2008Review
- Low rate of replication fork progression lengthens the replication timing of a locus containing an early firing origin.Nucleic acids research · 2007Article
- Replication-independent core histone dynamics at transcriptionally active loci in vivo.Genes & development · 2005Article
- The ribosomal RNA gene promoter and adjacent cis-acting DNA sequences govern plasmid DNA partitioning and stable inheritance in the parasitic protozoan Leishmania.Nucleic acids research · 2004Article
- Developmentally regulated usage of Physarum DNA replication origins.EMBO reports · 2003Article
- Replicational organization of three weakly expressed loci in Physarum polycephalum.Nucleic acids research · 2002Article
- Activity of the c-myc replicator at an ectopic chromosomal location.Molecular and cellular biology · 1999Article
- Short DNA fragments without sequence similarity are initiation sites for replication in the chromosome of the yeast Yarrowia lipolytica.Molecular biology of the cell · 1999Article
- Replication initiation and elongation fork rates within a differentially expressed human multicopy locus in early S phase.Nucleic acids research · 1997Article
- An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica.Molecular and cellular biology · 1997Article
- Characterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques.Molecular and cellular biology · 1996Article
- Article
- Mapping of a replication origin within the promoter region of two unlinked, abundantly transcribed actin genes of Physarum polycephalum.Molecular and cellular biology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Physarum polycephalum rRNA genes are found on extrachromosomal 60 kb linear palindromic DNA molecules. Previous work using electron microscope visualization suggested that these molecules are duplicated from one of four potential replication origins located in the 24 kb central non-transcribed spacer [Vogt and Braun (1977) Eur. J. Biochem., 80, 557-566]. Considering the controversy on the nature of the replication origins in eukaryotic cells, where both site-specific or delocalized initiations have been described, we study here Physarum rDNA replication by two dimensional agarose gel electrophoresis and compare the results to those obtained by electron microscopy. Without the need of cell treatment or enrichment in replication intermediates, we detect hybridization signals corresponding to replicating rDNA fragments throughout the cell cycle, confirming that the synthesis of rDNA molecules is not under the control of S-phase. The patterns of replication intermediates along rDNA minichromosomes are consistent with the existence of four site-specific replication origins, whose localization in the central non-transcribed spacer is in agreement with the electron microscope mapping. It is also shown that, on a few molecules, at least two origins are active simultaneously.
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