ArticleNucleic acids research1995
Presence of negative torsional tension in the promoter region of the transcriptionally poised dihydrofolate reductase gene in vivo.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 58 citations in OpenAlex.
- Apical localization of RNA polymerases modulates transcription dynamics and supercoiling domains revealed by cryo-ET.Molecular cell · 2026Article
- Apical Localization of RNA Polymerases Modulate Transcription Dynamics and Supercoiling Domains Revealed by Cryo-ET.bioRxiv : the preprint server for biology · 2026Article
- Histone Modifications, Internucleosome Dynamics, and DNA Stresses: How They Cooperate to "Functionalize" Nucleosomes.Frontiers in genetics · 2022Article
- Supercoiling and looping promote DNA base accessibility and coordination among distant sites.Nature communications · 2021Article
- Transcription-mediated supercoiling regulates genome folding and loop formation.Molecular cell · 2021Article
- Are TADs supercoiled?Nucleic acids research · 2019Review
- Investigating DNA supercoiling in eukaryotic genomes.Briefings in functional genomics · 2017Review
- On the role of inter-nucleosomal interactions and intrinsic nucleosome dynamics in chromatin function.Biochemistry and biophysics reports · 2016Review
- Effects of DNA supercoiling on chromatin architecture.Biophysical reviews · 2016Review
- Effects of DNA supercoiling on chromatin architecture.Biophysical reviews · 2016Review
- Profiling DNA supercoiling domains in vivo.Genomics data · 2014Article
- Chromatin regulates DNA torsional energy via topoisomerase II-mediated relaxation of positive supercoils.The EMBO journal · 2014Article
- Rate of elongation by RNA polymerase II is associated with specific gene features and epigenetic modifications.Genome research · 2014Article
- Transcription forms and remodels supercoiling domains unfolding large-scale chromatin structures.Nature structural & molecular biology · 2013Article
- Transcription-dependent dynamic supercoiling is a short-range genomic force.Nature structural & molecular biology · 2013Article
- Rad26, the transcription-coupled repair factor in yeast, is required for removal of stalled RNA polymerase-II following UV irradiation.PloS one · 2013Article
- The torsional state of DNA within the chromosome.Chromosoma · 2011Review
- A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding.Nucleic acids research · 2010Article
- Large-scale chromatin structure of inducible genes: transcription on a condensed, linear template.The Journal of cell biology · 2009Article
- Selective cleavage of AAVS1 substrates by the adeno-associated virus type 2 rep68 protein is dependent on topological and sequence constraints.Journal of virology · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA topology has been suggested to play an important role in the process of transcription. Negative torsional tension has been shown to stimulate both pre-initiation complex formation and promoter clearance on plasmid DNA in vitro. We recently showed that genomic DNA in human cells contains localized torsional tension. In the present study we have further characterized and mapped torsional tension in the dihydrofolate reductase (DHFR) gene in Chinese hamster ovary (CHO) cells and investigated the effects of differential rates of transcription on the magnitude and location of this tension. Using psoralen photo-cross-linking in conjunction with X-irradiation, we found that relaxable psoralen hypersensitivity was specifically localized to the promoter region of the serum-regulated DHFR gene in serum-stimulated, but not in serum-starved, cells. Moreover, this hypersensitivity did not appear to be caused by transcription elongation, since it persisted in cells in which transcription of the DHFR gene had been reduced by the transcription inhibitor 5,6-dichloro-1-beta-D-ribofurano-sylbenzimidazole (DRB). We suggest that the generation of negative torsional tension in DNA may play an important role in gene regulation by poising genes for transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.