ArticleNucleic acids research1995
Distamycin A and tallimustine inhibit TBP binding and basal in vitro transcription.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 62 citations in OpenAlex.
- Comparative in silico study of congocidine congeners as potential inhibitors of African swine fever virus.PloS one · 2019Article
- An overview of recent advances in duplex DNA recognition by small molecules.Beilstein journal of organic chemistry · 2018Review
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- Article
- Probing TBP interactions in transcription initiation and reinitiation with RNA aptamers that act in distinct modes.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Cloning and characterization of the 5'-flanking region of the oxalate decarboxylase gene from Flammulina velutipes.The Biochemical journal · 2002Article
- Inhibition of transcription factor-DNA complexes and gene expression by a microgonotropen.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Transcriptional activation by LR1 at the Emu enhancer and switch region sites.Nucleic acids research · 2000Article
- Interference of transcriptional activation by the antineoplastic drug ecteinascidin-743.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Chemical approaches to control gene expression.Gene expression · 2000Review
- Human T-cell leukemia virus type 1 Tax requires direct access to DNA for recruitment of CREB binding protein to the viral promoter.Molecular and cellular biology · 1998Article
- Characterization of a protein recognizing minor groove binders-damaged DNA.Nucleic acids research · 1996Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The antibiotic distamycin A is a DNA minor groove binding drug (MGB) that recognizes a stretch of at least four ATs. The alkylating benzoyl mustard derivative tallimustine (FCE 24517) has powerful anti-tumor activity. Using the electrophoretic mobility shift assay (EMSA) we determined that both compounds can prevent binding of TBP and, with 10-fold higher concentration, TBP-TFIIA (DA) and TBP-TFIIA-TFIIB (DAB) to a TATA box. Once formed, the DA and DAB complexes are more resistant to MGB challenge. Both drugs can inhibit basal in vitro transcription of a minimal TATA-containing promoter and similar concentrations are necessary for binding and transcriptional inhibition. Tallimustine shows strong selectivity by decreasing only correctly initiated transcripts. Even at high doses (20 microM), however, they cannot disturb a competent pre-initiation complex or Pol II progression. This functional in vitro model will provide a way to investigate the activity of sequence-specific DNA binding drugs with potential anti-viral and anti-tumour activity and to develop novel more selective compounds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.