ArticleThe EMBO journal1995
Extensive interactions of PRP8 protein with the 5' and 3' splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
96 citing papers in PubMed, 167 citations in OpenAlex.
- Swt21p Is Required for Nam8p-U1 snRNP Association and Efficient Pre-mRNA Splicing inInternational journal of molecular sciences · 2025Article
- psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation.Nature communications · 2021Article
- Regulation and Function of RNA Pseudouridylation in Human Cells.Annual review of genetics · 2020Review
- Functional analysis of Cwc24 ZF-domain in 5' splice site selection.Nucleic acids research · 2019Article
- Dynamic protein-RNA interactions in mediating splicing catalysis.Nucleic acids research · 2019Article
- Exon Junction Complex Shapes the Transcriptome by Repressing Recursive Splicing.Molecular cell · 2018Article
- Role of Cwc24 in the First Catalytic Step of Splicing and Fidelity of 5' Splice Site Selection.Molecular and cellular biology · 2017Article
- Regulation of constitutive and alternative mRNA splicing across the human transcriptome by PRPF8 is determined by 5' splice site strength.Genome biology · 2015Article
- Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.PLoS genetics · 2015Article
- Group II intron lariat: Structural insights into the spliceosome.RNA biology · 2015Review
- Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system.RNA (New York, N.Y.) · 2013Article
- Rearrangements within human spliceosomes captured after exon ligation.RNA (New York, N.Y.) · 2013Article
- Article
- Emerging views about the molecular structure of the spliceosomal catalytic center.RNA biology · 2012Article
- Brr2p-mediated conformational rearrangements in the spliceosome during activation and substrate repositioning.Genes & development · 2012Article
- Functional roles of protein splicing factors.Bioscience reports · 2012Review
- Saccharomyces cerevisiae NineTeen complex (NTC)-associated factor Bud31/Ycr063w assembles on precatalytic spliceosomes and improves first and second step pre-mRNA splicing efficiency.The Journal of biological chemistry · 2012Article
- Exon sequences at the splice junctions affect splicing fidelity and alternative splicing.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Mutations in the U5 snRNA result in altered splicing of subsets of pre-mRNAs and reduced stability of Prp8.RNA (New York, N.Y.) · 2009Article
- Splicing of mRNA precursors: the role of RNAs and proteins in catalysis.Molecular bioSystems · 2009Review
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precursor RNAs containing 4-thiouridine at specific sites were used with UV-crosslinking to map the binding sites of the yeast protein splicing factor PRP8. PRP8 protein interacts with a region of at least eight exon nucleotides at the 5' splice site and a minimum of 13 exon nucleotides and part of the polypyrimidine tract in the 3' splice site region. Crosslinking of PRP8 to mutant and duplicated 3' splice sites indicated that the interaction is not sequence specific, nor does it depend on the splice site being functional. Binding of PRP8 to the 5' exon was established before step 1 and to the 3' splice site region after step 1 of splicing. These interactions place PRP8 close to the proposed catalytic core of the spliceosome during both transesterification reactions. To date, this represents the most extensive mapping of the binding site(s) of a splicing factor on the substrate RNA. We propose that the large binding sites of PRP8 stabilize the intrinsically weaker interactions of U5 snRNA with both exons at the splice sites for exon alignment by the U5 snRNP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.