ArticleThe EMBO journal1995
A component of the chloroplastic protein import apparatus is targeted to the outer envelope membrane via a novel pathway.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
79 citing papers in PubMed, 219 citations in OpenAlex.
- Revealing Missing Links in the Downsizing of the Photosystem II Antenna in Higher Plants Under Stress Conditions.Antioxidants (Basel, Switzerland) · 2025Article
- Genetic Mapping of theFrontiers in plant science · 2022Article
- Chloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic236.Plant direct · 2021Article
- Article
- Insertion of plastidic β-barrel proteins into the outer envelopes of plastids involves an intermembrane space intermediate formed with Toc75-V/OEP80.The Plant cell · 2021Article
- Toc75-V/OEP80 is processed during translocation into chloroplasts, and the membrane-embedded form exposes its POTRA domain to the intermembrane space.FEBS open bio · 2020Article
- Chloroplast Outer Membrane β-Barrel Proteins Use Components of the General Import Apparatus.The Plant cell · 2019Article
- Differentiation of chromoplasts and other plastids in plants.Plant cell reports · 2019Review
- A Protochlorophyllide (Pchlide)Frontiers in plant science · 2019Article
- Evolution of protein transport to the chloroplast envelope membranes.Photosynthesis research · 2018Review
- Import of Soluble Proteins into Chloroplasts and Potential Regulatory Mechanisms.Frontiers in plant science · 2017Review
- Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts.Plant physiology · 2016Article
- Polyglycine Acts as a Rejection Signal for Protein Transport at the Chloroplast Envelope.PloS one · 2016Article
- New insights into the mechanism of chloroplast protein import and its integration with protein quality control, organelle biogenesis and development.Journal of molecular biology · 2015Review
- Two activities of long-chain acyl-coenzyme A synthetase are involved in lipid trafficking between the endoplasmic reticulum and the plastid in Arabidopsis.Plant physiology · 2015Article
- Targeting and assembly of components of the TOC protein import complex at the chloroplast outer envelope membrane.Frontiers in plant science · 2014Review
- The C-terminus of Bienertia sinuspersici Toc159 contains essential elements for its targeting and anchorage to the chloroplast outer membrane.Frontiers in plant science · 2014Article
- Evolution and targeting of Omp85 homologs in the chloroplast outer envelope membrane.Frontiers in plant science · 2014Article
- The selective biotin tagging and thermolysin proteolysis of chloroplast outer envelope proteins reveals information on protein topology and association into complexes.Frontiers in plant science · 2014Article
- Probing Arabidopsis chloroplast diacylglycerol pools by selectively targeting bacterial diacylglycerol kinase to suborganellar membranes.Plant physiology · 2013Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A chloroplastic outer envelope membrane protein of 75 kDa (OEP75) was identified previously as a component of the protein import machinery. Here we provide additional evidence that OEP75 is a component of protein import, present the isolation of a cDNA clone encoding this protein, briefly describe its developmental expression and tissue specificity, and characterize its insertion into the outer envelope membrane. OEP75 was synthesized as a higher molecular weight precursor (prOEP75) which bound to isolated chloroplasts in an in vitro import assay and subsequently was processed to the mature form (mOEP75). During this import assay, two proteins intermediate in size between prOEP75 and mOEP75 were detected. One of these intermediates was also detected in chloroplast envelopes isolated from young pea leaves. Binding and processing of prOEP75 required ATP and one or more surface-exposed proteinaceous components, and was competed by prSSU, a stromal-targeted protein. We propose that the N-terminus of the prOEP75 transit peptide acts as a stromal-targeting domain and a central, hydrophobic region of this transit peptide acts as a stop-transfer domain. A complex route of insertion and processing of prOEP75 may exist to ensure high fidelity targeting of this import component.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.