ArticleThe American journal of pathology1995
Characterization and functional studies on rat liver fat-storing cell line and freshly isolated hepatocyte coculture system.
Article in The American journal of pathology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 85 citations in OpenAlex.
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- Recent Advances in Body-on-a-Chip Systems.Analytical chemistry · 2019Review
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- Connexins and pannexins in liver damage.EXCLI journal · 2016Review
- Connexin and pannexin signaling in gastrointestinal and liver disease.Translational research : the journal of laboratory and clinical medicine · 2015Review
- Cell sources, liver support systems and liver tissue engineering: alternatives to liver transplantation.International journal of stem cells · 2015Review
- Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes.International journal of medical sciences · 2015Article
- Cell and tissue engineering for liver disease.Science translational medicine · 2014Review
- The synthetic hydroxyproline-containing collagen analogue (Gly-Pro-Hyp)10 promotes enzymatic activity of matrixmetalloproteinase-2 in vitro.European journal of microbiology & immunology · 2012Article
- Organotypic liver culture models: meeting current challenges in toxicity testing.Critical reviews in toxicology · 2012Review
- Cannabinoid receptor type I modulates alcohol-induced liver fibrosis.Molecular medicine (Cambridge, Mass.) · 2011Article
- Co-culture of primary rat hepatocytes with rat liver epithelial cells enhances interleukin-6-induced acute-phase protein response.Cell and tissue research · 2010Article
- Role of the receptor for advanced glycation end products in hepatic fibrosis.World journal of gastroenterology · 2009Article
- Hedgehog pathway activation and epithelial-to-mesenchymal transitions during myofibroblastic transformation of rat hepatic cells in culture and cirrhosis.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Liver tissue engineering in the evaluation of drug safety.Expert opinion on drug metabolism & toxicology · 2009Review
- Mesenchymal stromal cells as supportive cells for hepatocytes.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Review
- Amino acid-mediated heterotypic interaction governs performance of a hepatic tissue model.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- T-cadherin modulates hepatocyte functions in vitro.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- Three-dimensional co-culture of hepatocytes and stellate cells.Cytotechnology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
We developed and characterized a coculture system composed of a fat-storing cell clone (CFSC-2G) and freshly isolated hepatocytes that can reproduce in vitro some of the physical and functional relationships observed in vivo. Hepatocytes in the coculture are polarized, are smaller in size than hepatocytes plated on plastic, maintain a cuboidal shape, and have a tendency to form cords. Fat-storing cells, which are initially extended, retract and leave spaces that resemble liver sinusoids. Both cell types in the coculture system are functional for at least two weeks as determined by the expression of high levels of liver-specific protein mRNAs as well as by the production and secretion of liver-specific proteins into the culture medium. The hepatocytes maintain relatively high levels of asialoglycoprotein receptor on their cell surface and form functional gap junctional complexes with fat-storing cells. Hence, this coculture system retains a number of differentiated functions of hepatocytes, making it a useful model to study cell-cell interactions in culture and to analyze regulation of hepatocyte functions.
Indexed as
Identifiers
7778689PMC1870907W1241851957What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.