ArticleProceedings of the National Academy of Sciences of the United States of America1995
Transient transfection and expression of firefly luciferase in Giardia lamblia.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 83 citations in OpenAlex.
- Harnessing the power of new genetic tools to illuminate Giardia biology and pathogenesis.Genetics · 2024Review
- Analysis of the role of acetylation inFrontiers in microbiology · 2024Article
- Antibodies to variable surface antigens induce antigenic variation in the intestinal parasite Giardia lamblia.Nature communications · 2023Article
- Efficient CRISPR/Cas9-mediated gene disruption in the tetraploid protistOpen biology · 2022Article
- Recent advances in functional research in Giardia intestinalis.Advances in parasitology · 2020Review
- Transfection With Plasmid Causing Stable Expression of a Foreign Gene Affects General Proteome Pattern inFrontiers in cellular and infection microbiology · 2020Article
- Successful Genetic Transfection of the Colonic Protistan Parasite Blastocystis for Reliable Expression of Ectopic Genes.Scientific reports · 2019Article
- Article
- Calcium-binding proteins that are type B″ regulatory subunits of phosphatase 2A in Giardia intestinalis.Parasitology research · 2018Article
- Article
- Giardia's primitive GPL biosynthesis pathways with parasitic adaptation 'patches': implications for Giardia's evolutionary history and for finding targets against Giardiasis.Scientific reports · 2017Article
- Interactions between Giardia duodenalis Sm proteins and their association with spliceosomal snRNAs.Parasitology research · 2017Article
- Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia.Nature communications · 2016Article
- Identification of Giardia lamblia DHHC proteins and the role of protein S-palmitoylation in the encystation process.PLoS neglected tropical diseases · 2014Article
- The giardial VPS35 retromer subunit is necessary for multimeric complex assembly and interaction with the vacuolar protein sorting receptor.Biochimica et biophysica acta · 2013Article
- SUMOylation in Giardia lamblia: A Conserved Post-Translational Modification in One of the Earliest Divergent Eukaryotes.Biomolecules · 2012Article
- Vacuolar protein sorting receptor in Giardia lamblia.PloS one · 2012Article
- Giardia lamblia low-density lipoprotein receptor-related protein is involved in selective lipoprotein endocytosis and parasite replication.Molecular microbiology · 2011Article
- Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia.The Journal of biological chemistry · 2008Article
- Arginine deiminase has multiple regulatory roles in the biology of Giardia lamblia.Journal of cell science · 2008Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a gene transfer system for the protozoan parasite Giardia lamblia. This organism is responsible for many cases of diarrhea worldwide and is considered to be one of the most primitive eukaryotes. Expression of a heterologous gene was detected in this parasite after electroporation with appropriate DNA constructs. We constructed a series of transfection plasmids using flanking sequences of the Giardia glutamate dehydrogenase (GDH) gene to drive expression of the firefly luciferase reporter gene. The optimal construct consisted of a GDH/luciferase fusion gene in which the first 18 codons of the GDH gene immediately preceded the luciferase gene; this fusion gene was flanked by the upstream and downstream sequences of the GDH gene. Electroporation of this construct into Giardia yielded luciferase activity that was 3000- to 50,000-fold above background. Removal of either the 5' or 3' GDH flanking sequences from this construct resulted in significantly reduced luciferase activity, and removal of both flanking sequences reduced luciferase activity to background levels. Luciferase activity was proportional to the amount of DNA electroporated and was maximal at 6 hr after electroporation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.