ArticleProceedings of the National Academy of Sciences of the United States of America1995
Genomic DNA fingerprinting by restriction fragment end labeling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 51 citations in OpenAlex.
- Molecular epidemiology of pneumococcal colonization in response to pneumococcal conjugate vaccination in children with recurrent acute otitis media.Journal of clinical microbiology · 2005Trial
- Application of molecular techniques to the study of hospital infection.Clinical microbiology reviews · 2006Review
- Molecular epidemiology of penicillin-susceptible non-beta-lactam-resistant Streptococcus pneumoniae isolates from Greek children.Journal of clinical microbiology · 2003Article
- Molecular epidemiology of pneumococcal carriage among children with upper respiratory tract infections in Hanoi, Vietnam.Journal of clinical microbiology · 2002Article
- Pneumococcal carriage in children in The Netherlands: a molecular epidemiological study.Journal of clinical microbiology · 2001Article
- Molecular epidemiology of penicillin-susceptible, multidrug-resistant serotype 6B pneumococci isolated from children in Greece.Journal of clinical microbiology · 2001Article
- Molecular epidemiology of penicillin-nonsusceptible Streptococcus pneumoniae among children in Greece.Journal of clinical microbiology · 2000Article
- Genetic relatedness within serotypes of penicillin-susceptible Streptococcus pneumoniae isolates.Journal of clinical microbiology · 2000Article
- Comparison of methods based on different molecular epidemiological markers for typing of Mycobacterium tuberculosis complex strains: interlaboratory study of discriminatory power and reproducibility.Journal of clinical microbiology · 1999Article
- Multidrug-resistant Streptococcus pneumoniae in Poland: identification of emerging clones.Journal of clinical microbiology · 1999Article
- Nasopharyngeal carriage of penicillin-resistant Streptococcus pneumoniae among children with acute respiratory tract infections in Thailand: a molecular epidemiological survey.Journal of clinical microbiology · 1999Article
- Molecular characterization of pneumococcal nasopharynx isolates collected from children during their first 2 years of life.Journal of clinical microbiology · 1998Article
- DNA fingerprinting techniques for microorganisms. A proposal for classification and nomenclature.Molecular biotechnology · 1996Review
- Molecular epidemiological characteristics of pneumococcal meningitis in children.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A typing method for bacteria was developed and applied to several species, including Escherichia coli and Actinobacillus actinomycetemcomitans. Total genomic DNA was digested with a restriction endonuclease, and fragments were enabled with [alpha-32P]dATP by using the Klenow fragment of DNA polymerase and separated by electrophoresis in 6% polyacrylamide/8 M urea (sequencing gel). Depending on the restriction endonuclease and the bacterium, the method produced approximately 30-50 well-separated fragments in the size range of 100-400 nucleotides. For A. actinomycetemcomitans, all strains had bands in common. Nevertheless, many polymorphisms could be observed, and the 31 strains tested could be classified into 29 distinct types. Furthermore, serotype-specific fragments could be assigned for the three serotypes investigated. The method described is very sensitive, allowing more distinct types to be distinguished than other commonly used typing methods. When the method was applied to 10 other clinically relevant bacterial species, both species-specific bands and strain-specific bands were found. Isolates from different locations of one patient showed indistinguishable patterns. Computer-assisted analysis of the DNA fingerprints allowed the determination of similarity coefficients. It is concluded that genomic fingerprinting by restriction fragment end labeling (RFEL) is a powerful and generally applicable technique to type bacterial species.
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