ArticleProceedings of the National Academy of Sciences of the United States of America1995
Chromosomal insertion of foreign (adenovirus type 12, plasmid, or bacteriophage lambda) DNA is associated with enhanced methylation of cellular DNA segments.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 121 citations in OpenAlex.
- Adenoviral Vector DNA- and SARS-CoV-2 mRNA-Based Covid-19 Vaccines: Possible Integration into the Human Genome - Are Adenoviral Genes Expressed in Vector-based Vaccines?Virus research · 2021Review
- Plasmid expression level heterogeneity monitoring via heterologous eGFP production at the single-cell level in Cupriavidus necator.Applied microbiology and biotechnology · 2020Article
- Essential concepts are missing: Foreign DNA in food invades the organisms' cells and can lead to stochastic epigenetic alterations with a wide range of possible pathogenetic consequences.Clinical epigenetics · 2020Article
- Evidence for Tethering of Human Cytomegalovirus Genomes to Host Chromosomes.Frontiers in cellular and infection microbiology · 2020Article
- Epigenetics and the dynamics of chromatin during adenovirus infections.FEBS letters · 2019Review
- Detecting Large Chromosomal Modifications Using Short Read Data From Genotyping-by-Sequencing.Frontiers in plant science · 2019Article
- Inheritable epigenetic response towards foreign DNA entry by mammalian host cells: a guardian of genomic stability.Epigenetics · 2018Review
- Viral epigenetics.Methods in molecular biology (Clifton, N.J.) · 2015Review
- HIV-1 Tat induces DNMT over-expression through microRNA dysregulation in HIV-related non Hodgkin lymphomas.Infectious agents and cancer · 2014Article
- DNA methylation mediated control of gene expression is critical for development of crown gall tumors.PLoS genetics · 2013Article
- Extensive microsatellite variation in rice induced by introgression from wild rice (Zizania latifolia Griseb.).PloS one · 2013Article
- Beyond genetics: epigenetic code in chronic kidney disease.Kidney international · 2011Review
- Unintended consequence of plant transformation: biolistic transformation caused transpositional activation of an endogenous retrotransposon Tos17 in rice ssp. japonica cv. Matsumae.Plant cell reports · 2009Article
- Epigenetic mechanisms in human adenovirus type 12 oncogenesis.Seminars in cancer biology · 2009Review
- Molecular characterization of a rice mutator-phenotype derived from an incompatible cross-pollination reveals transgenerational mobilization of multiple transposable elements and extensive epigenetic instability.BMC plant biology · 2009Article
- Erythroid-specific expression of beta-globin by the sleeping beauty transposon for Sickle cell disease.Biochemistry · 2007Article
- Epigenetic status of an adenovirus type 12 transgenome upon long-term cultivation in hamster cells.Journal of virology · 2007Article
- Extent and pattern of DNA methylation alteration in rice lines derived from introgressive hybridization of rice and Zizania latifolia Griseb.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Human polyomavirus BKV transcriptionally activates DNA methyltransferase 1 through the pRb/E2F pathway.Oncogene · 2006Article
- Extensive de Novo genomic variation in rice induced by introgression from wild rice (Zizania latifolia Griseb.).Genetics · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insertion of foreign DNA into an established mammalian genome can extensively alter the patterns of cellular DNA methylation. Adenovirus type 12 (Ad12)-transformed hamster cells, Ad12-induced hamster tumor cells, or hamster cells carrying integrated DNA of bacteriophage lambda were used as model systems. DNA methylation levels were examined by cleaving cellular DNA with Hpa II, Msp I, or Hha I, followed by Southern blot hybridization with 32P-labeled, randomly selected cellular DNA probes. For several, but not all, cellular DNA segments investigated, extensive increases in DNA methylation were found in comparison with the methylation patterns in BHK21 or primary Syrian hamster cells. In eight different Ad12-induced hamster tumors, moderate increases in DNA methylation were seen. Increased methylation of cellular genes was also documented in two hamster cell lines with integrated Ad12 DNA without the Ad12-transformed phenotype, in one cloned BHK21 cell line with integrated plasmid DNA, and in at least three cloned BHK21 cell lines with integrated lambda DNA. By fluorescent in situ hybridization, the cellular hybridization probes were located to different hamster chromosomes. The endogenous intracisternal A particle genomes showed a striking distribution on many hamster chromosomes, frequently on their short arms. When BHK21 hamster cells were abortively infected with Ad12, increases in cellular DNA methylation were not seen. Thus, Ad12 early gene products were not directly involved in increasing cellular DNA methylation. We attribute the alterations in cellular DNA methylation, at least in part, to the insertion of foreign DNA. Can alterations in the methylation profiles of hamster cellular DNA contribute to the generation of the oncogenic phenotype?
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