ArticleThe Journal of cell biology1995
The impact of chromosomes and centrosomes on spindle assembly as observed in living cells.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 95 citations in OpenAlex.
- An Arabidopsis Kinesin-14D motor is associated with midzone microtubules for spindle morphogenesis.Current biology : CB · 2024Article
- Micromanipulation of Chromosomes in Insect Spermatocytes.Journal of visualized experiments : JoVE · 2018Article
- Tension sensors reveal how the kinetochore shares its load.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Assembly of microtubules and actomyosin rings in the absence of nuclei and spindle pole bodies revealed by a novel genetic method.PloS one · 2007Article
- The mechanism of spindle assembly: functions of Ran and its target TPX2.The Journal of cell biology · 2004Review
- Micromechanical studies of mitotic chromosomes.Journal of muscle research and cell motility · 2002Review
- Male meiotic spindle lengths in normal and mutant arabidopsis cells.Plant physiology · 2001Article
- Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix.The Journal of cell biology · 2000Article
- Mutation of a Drosophila gamma tubulin ring complex subunit encoded by discs degenerate-4 differentially disrupts centrosomal protein localization.Genes & development · 2000Article
- Nuclear gamma-tubulin during acentriolar plant mitosis.The Plant cell · 2000Article
- Cytoskeleton: centrosom-in absentia.Current biology : CB · 1999Review
- The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle.The Journal of cell biology · 1999Article
- Slk19p is a centromere protein that functions to stabilize mitotic spindles.The Journal of cell biology · 1999Article
- Kinetochore fibers are not involved in the formation of the first meiotic spindle in mouse oocytes, but control the exit from the first meiotic M phase.The Journal of cell biology · 1999Article
- Chromosomal influence on meiotic spindle assembly: abnormal meiosis I in female Mlh1 mutant mice.The Journal of cell biology · 1999Article
- AIR-2: An Aurora/Ipl1-related protein kinase associated with chromosomes and midbody microtubules is required for polar body extrusion and cytokinesis in Caenorhabditis elegans embryos.The Journal of cell biology · 1998Article
- Spindle self-organization and cytokinesis during male meiosis in asterless mutants of Drosophila melanogaster.The Journal of cell biology · 1998Article
- Spindle assembly and mitosis without centrosomes in parthenogenetic Sciara embryos.The Journal of cell biology · 1998Article
- Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis.The EMBO journal · 1997Article
- Mitotic spindle poles are organized by structural and motor proteins in addition to centrosomes.The Journal of cell biology · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We analyzed the role that chromosomes, kinetochores, and centrosomes play in spindle assembly in living grasshopper spermatocytes by reconstructing spindles lacking certain components. We used video-enhanced, polarization microscopy to distinguish the effect of each component on spindle microtubule dynamics and we discovered that both chromosomes and centrosomes make potent and very different contributions to the organization of the spindle. Remarkably, the position of a single chromosome can markedly affect the distribution of microtubules within a spindle or even alter the fate of spindle assembly. In an experimentally constructed spindle having only one chromosome, moving the chromosome to one of the two poles induces a dramatic assembly of microtubules at the nearer pole and a concomitant disassembly at the farther pole. So long as a spindle carries a single chromosome it will persist normally. A spindle will also persist even when all chromosomes are detached and then removed from the cell. If, however, a single chromosome remains in the cell but is detached from the spindle and kept in the cytoplasm, the spindle disassembles. One might expect the effect of chromosomes on spindle assembly to relate to a property of a specific site on each chromosome, perhaps the kinetochore. We have ruled out that possibility by showing that it is the size of chromosomes rather than the number of kinetochores that matters. Although chromosomes affect spindle assembly, they cannot organize a spindle in the absence of centrosomes. In contrast, centrosomes can organize a functional bipolar spindle in the absence of chromosomes. If both centrosomes and chromosomes are removed from the cell, the spindle quickly disappears.
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