ArticleThe Biochemical journal1995
Differential localization of the mRNA of the M and B isoforms of creatine kinase in myoblasts.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 50 citations in OpenAlex.
- The Genetic Basis of Non-Contact Soft Tissue Injuries-Are There Practical Applications of Genetic Knowledge?Cells · 2024Review
- Ancestral contribution of the muscle-specific creatine kinase (CKM) polymorphism rs4884 in the knee osteoarthritis risk: a preliminary study.Clinical rheumatology · 2021Article
- A meta-analysis of the association of CKM gene rs8111989 polymorphism with sport performance.Biology of sport · 2017Article
- Genetic variation and exercise-induced muscle damage: implications for athletic performance, injury and ageing.European journal of applied physiology · 2016Review
- Evaluation of a 7-Gene Genetic Profile for Athletic Endurance Phenotype in Ironman Championship Triathletes.PloS one · 2015Article
- Association of Plasma Heat Shock Protein 70, Interleukin 6, and Creatine Kinase Concentrations in a Healthy, Young Adult Population.Journal of biomarkers · 2015Article
- Genetic polymorphisms associated with exertional rhabdomyolysis.European journal of applied physiology · 2013Article
- Muscle-specific creatine kinase gene polymorphism and running economy responses to an 18-week 5000-m training programme.British journal of sports medicine · 2006Article
- Elongation factor 1alpha binds to the region of the metallothionein-1 mRNA implicated in perinuclear localization--importance of an internal stem-loop.RNA (New York, N.Y.) · 2006Article
- An AU-rich stem-loop structure is a critical feature of the perinuclear localization signal of c-myc mRNA.The Biochemical journal · 2005Article
- Perinuclear localization of slow troponin C m RNA in muscle cells is controlled by a cis-element located at its 3' untranslated region.RNA (New York, N.Y.) · 2005Article
- Role of creatine kinase isoenzymes on muscular and cardiorespiratory endurance: genetic and molecular evidence.Sports medicine (Auckland, N.Z.) · 2001Review
- Competition between the signal sequence and a 3'UTR localisation signal during redirection of beta-globin mRNA to the endoplasmic reticulum: implications for biotechnology.Cytotechnology · 1999Article
- A human sequence homologue of Staufen is an RNA-binding protein that is associated with polysomes and localizes to the rough endoplasmic reticulum.Molecular and cellular biology · 1999Article
- Arginine kinase expression and localization in growth cone migration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- RNA-protein interactions within the 3 ' untranslated region of vimentin mRNA.Nucleic acids research · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Creatine kinase (CK) plays an important role in buffering ATP and ADP levels in tissues which have intermittently high and fluctuating energy demands, such as skeletal muscle. This buffering function has a spatial, as well as a temporal aspect, which is dependent on the localization of different enzyme isoforms within the cell. We show here, by in situ hybridization, that the mRNAs for the cytoplasmic isoforms of CK are differentially localized in a mouse myoblast cell line (C2C12). The mRNA for the M form is localized at the cell periphery, while that for the B form is localized in the perinuclear region. Deletion of segments of the 3' untranslated regions of these mRNAs or swapping of these segments between the mRNAs for the two isoforms demonstrated that localization signals lie within these regions. Localization appears to be tissue-specific, since both the M and B mRNAs were distributed uniformly over the cytoplasm in a non-muscle cell line. These results, in conjunction with other studies which have shown that mRNA localization can lead to co-localization of the encoded protein, suggest that the localization of the mRNAs for the cytoplasmic isoforms of CK may be involved in the localization of the enzymes themselves.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.