ArticleThe Biochemical journal1995
Murine protein tyrosine phosphatase-PEST, a stable cytosolic protein tyrosine phosphatase.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- Regulation of TCR signalling by tyrosine phosphatases: from immune homeostasis to autoimmunity.Immunology · 2012Pooled it
- Regulation of the Src kinase-associated phosphoprotein 55 homologue by the protein tyrosine phosphatase PTP-PEST in the control of cell motility.The Journal of biological chemistry · 2013Article
- The phosphatase PTP-PEST/PTPN12 regulates endothelial cell migration and adhesion, but not permeability, and controls vascular development and embryonic viability.The Journal of biological chemistry · 2012Article
- Podocyte Protein, Nephrin, Is a Substrate of Protein Tyrosine Phosphatase 1B.Journal of signal transduction · 2011Article
- Caspase-3 regulates catalytic activity and scaffolding functions of the protein tyrosine phosphatase PEST, a novel modulator of the apoptotic response.Molecular and cellular biology · 2007Article
- Elicitor-mediated oligomerization of the tobacco N disease resistance protein.The Plant cell · 2006Article
- Protein tyrosine phosphatase 1B attenuates growth hormone-mediated JAK2-STAT signaling.Molecular and cellular biology · 2003Article
- Paxillin: a focal adhesion-associated adaptor protein.Oncogene · 2001Review
- Products of US22 genes M140 and M141 confer efficient replication of murine cytomegalovirus in macrophages and spleen.Journal of virology · 2001Article
- The tumor-suppressor activity of PTEN is regulated by its carboxyl-terminal region.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Protein tyrosine phosphatase-PEST regulates focal adhesion disassembly, migration, and cytokinesis in fibroblasts.The Journal of cell biology · 1999Article
- The murine Sim-2 gene product inhibits transcription by active repression and functional interference.Molecular and cellular biology · 1997Article
- PSTPIP: a tyrosine phosphorylated cleavage furrow-associated protein that is a substrate for a PEST tyrosine phosphatase.The Journal of cell biology · 1997Article
- Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST.Molecular and cellular biology · 1996Article
- Association of inhibitory tyrosine protein kinase p50csk with protein tyrosine phosphatase PEP in T cells and other hemopoietic cells.The EMBO journal · 1996Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated the murine cDNA homologue of the human protein tyrosine phosphatase PTP-PEST (MPTP-PEST) from an 18.5-day mouse embryonic kidney library. The cDNA isolated has a single open reading frame predicting a protein of 775 amino acids. When expressed in vitro as a glutathione S-transferase fusion protein, the catalytic domain (residues 1-453) shows intrinsic phosphatase activity. Reverse transcriptase PCR and Northern-blot analysis show that MPTP-PEST mRNA is expressed throughout murine development. Indirect immunofluorescence in COS-1 cells against a heterologous epitope tag attached to the N-terminus of MPTP-PEST, together with cellular fractionation and Western-blot experiments from different murine cell lines, indicate that MPTP-PEST is a free cytosolic protein of 112 kDa. Finally, sequence analysis indicates that the C-terminal portion of the protein contains four regions rich in proline, glutamate, serine and threonine, otherwise known as PEST sequences. These are characteristic of proteins that display very short intracellular half-lives. Despite the presence of these motifs, pulse-chase labelling experiments demonstrate that MPTP-PEST has a half-life of more than 4 h.
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