ArticleThe Journal of clinical investigation1995
Heat shock induces resistance in rat pancreatic islet cells against nitric oxide, oxygen radicals and streptozotocin toxicity in vitro.
Article in The Journal of clinical investigation, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 154 citations in OpenAlex.
- Diabetes, Protein Misfolding, and Heat Stress: Molecular Insights and Translational Perspectives.TH open : companion journal to thrombosis and haemostasis · 2026Review
- Defining Cytokine Responsive and Non-responsive Human β-cells.Function (Oxford, England) · 2024Article
- Single Cell RNAseq Analysis of Cytokine-Treated Human Islets: Association of Cellular Stress with Impaired Cytokine Responsiveness.Function (Oxford, England) · 2024Article
- β-Cell-selective regulation of gene expression by nitric oxide.American journal of physiology. Regulatory, integrative and comparative physiology · 2024Article
- Single-cell RNA sequencing of mouse islets exposed to proinflammatory cytokines.Life science alliance · 2021Article
- Participation of Hsp70 and Hsp90α Heat Shock Proteins in Stress Response in the Course of Type 1 Diabetes Mellitus.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2020Article
- The Role of Heat Shock Proteins in Type 1 Diabetes.Frontiers in immunology · 2020Review
- Promoting Neuronal Tolerance of Diabetic Stress: Modulating Molecular Chaperones.International review of neurobiology · 2016Review
- Heat shock protein 70 (Hsp70) inhibits oxidative phosphorylation and compensates ATP balance through enhanced glycolytic activity.Journal of applied physiology (Bethesda, Md. : 1985) · 2012Article
- The effect of smoking cessation pharmacotherapies on pancreatic beta cell function.Toxicology and applied pharmacology · 2012Article
- KU-32, a novel drug for diabetic neuropathy, is safe for human islets and improves in vitro insulin secretion and viability.Experimental diabetes research · 2012Article
- Islet transplantation and encapsulation: an update on recent developments.The review of diabetic studies : RDS · 2011Review
- Role of the mitochondria in immune-mediated apoptotic death of the human pancreatic β cell line βLox5.PloS one · 2011Article
- Innate immunity and heat shock response in islet transplantation.Clinical and experimental immunology · 2009Review
- Regulation of insulin secretion, glucokinase gene transcription and beta cell proliferation by adipocyte-derived Wnt signalling molecules.Diabetologia · 2008Article
- A protective role for heme oxygenase-1 in INS-1 cells and rat islets that are exposed to high glucose conditions.Journal of Korean medical science · 2006Article
- Mild whole-body heat stress alters retinoid metabolism in the rat small intestine.Digestive diseases and sciences · 2006Article
- Heat shock protein 70 and the acute respiratory distress syndrome.Journal of anesthesia · 2005Review
- Clonogenicity of human leukemic cells protected from cell-lethal agents by heat shock protein 70.Cell stress & chaperones · 2005Article
- Protective effect of heat shock protein 70 against oxidative stresses in human corneal fibroblasts.Journal of Korean medical science · 2004Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When cultures of pancreatic islet cells are exposed to the nitric oxide donor sodium nitroprusside, to enzymatically generated reactive oxygen intermediates or to streptozotocin cell lysis occurs after 4-12 h. We report here that a heat shock at 43 degrees C for 90 min reduces cell lysis from nitric oxide (0.45 mM sodium nitroprusside) by 70%, from reactive oxygen intermediates (12 mU xanthine oxidase and 0.05 mM hypoxanthine) by 80% and from streptozotocin (1.5 mM) by 90%. Heat shock induced resistance was observed immediately after termination of the 90 min culture at 43 degrees C and correlated with enhanced expression of hsp70. The occurrence of DNA strand breaks, a major early consequence of nitric oxide, reactive oxygen intermediates, or streptozotocin action, was not suppressed by heat shock treatment. However, the depletion of NAD+, the major cause of radical induced islet cell death, was suppressed after heat shock (P < 0.01). We conclude that pancreatic islet cells can rapidly activate defence mechanisms against nitric oxide, reactive oxygen intermediates and streptozotocin by culture at 43 degrees C. Islet cell survival is due to the prevention of lethal NAD+ depletion during DNA repair, probably by slowing down poly(ADP-ribose)polymerase activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.