ArticleJournal of bacteriology1995
Energy buffering of DNA structure fails when Escherichia coli runs out of substrate.
Article in Journal of bacteriology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- E. coli prepares for starvation by dramatically remodeling its proteome in the first hours after loss of nutrients.Molecular systems biology · 2026Article
- Negative DNA supercoiling enhances DARS2 binding of DNA-bending protein IHF in the activation of Fis-dependent ATP-DnaA production.Nucleic acids research · 2025Article
- Energy Starvation Induces a Cell Cycle Arrest inFrontiers in molecular biosciences · 2021Article
- Article
- DNA supercoiling and transcription in bacteria: a two-way street.BMC molecular and cell biology · 2019Review
- Article
- Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.Biophysical reviews · 2016Review
- Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.Biophysical reviews · 2016Review
- DNA supercoiling, a critical signal regulating the basal expression of the lac operon in Escherichia coli.Scientific reports · 2016Article
- Direct Mg(2+) binding activates adenylate kinase from Escherichia coli.The Journal of biological chemistry · 2009Article
- Two nucleoside uptake systems in Lactococcus lactis: competition between purine nucleosides and cytidine allows for modulation of intracellular nucleotide pools.Journal of bacteriology · 2003Article
- Escherichia coli and Salmonella 2000: the view from here.Microbiology and molecular biology reviews : MMBR · 2001Review
- DNA topology and adaptation of Salmonella typhimurium to an intracellular environment.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2000Review
- Transcriptional coupling between the divergent promoters of a prototypic LysR-type regulatory system, the ilvYC operon of Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- atp Mutants of Escherichia coli fail to grow on succinate due to a transport deficiency.Journal of bacteriology · 1998Article
- DNA gyrase, topoisomerase IV, and the 4-quinolones.Microbiology and molecular biology reviews : MMBR · 1997Review
- Effects of sigmaS and the transcriptional activator AppY on induction of the Escherichia coli hya and cbdAB-appA operons in response to carbon and phosphate starvation.Journal of bacteriology · 1997Article
- Supercoil-induced extrusion of a regulatory DNA hairpin.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Identification of sigma S-regulated genes in Salmonella typhimurium: complementary regulatory interactions between sigma S and cyclic AMP receptor protein.Journal of bacteriology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study how changes in the [ATP]/[ADP] ratio affect the level of DNA supercoiling in Escherichia coli, the cellular content of H(+)-ATPase was modulated around the wild-type level. A relatively large drop in the [ATP]/[ADP] ratio from the normal ratio resulted in a small increase in the linking number of our reporter plasmid (corresponding to a small decrease in negative supercoiling). However, when cells depleted their carbon and energy source, the ensuing drop in energy state was accompanied by a strong increase in linking number. This increase was not due to reduced transcription of the DNA in the absence of growth substrate, since rifampin had virtually no effect on the plasmid linking number. To examine whether DNA supercoiling depends more strongly on the cellular energy state at low [ATP]/[ADP] ratios than at high ratios, we used cells that were already at a low energy state after substrate depletion; after the addition of an uncoupler to these cells, the [ATP]/[ADP] ratio decreased further, which resulted in a strong increase in plasmid linking number. Our results suggest that the strong thermodynamic control of DNA supercoiling takes over at low [ATP]/[ADP] ratios, whereas at high ratios homeostatic control mechanisms attenuate thermodynamic control.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.