ArticleApplied microbiology and biotechnology1993
Molecular cloning, expression and structure of the endo-1,5-alpha-L-arabinase gene of Aspergillus niger.
Article in Applied microbiology and biotechnology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 57 citations in OpenAlex.
- SACCHARIS: an automated pipeline to streamline discovery of carbohydrate active enzyme activities within polyspecific families and de novo sequence datasets.Biotechnology for biofuels · 2018Article
- Expression-based clustering of CAZyme-encoding genes of Aspergillus niger.BMC genomics · 2017Article
- Enzymatic Mechanism for Arabinan Degradation and Transport in the Thermophilic Bacterium Caldanaerobius polysaccharolyticus.Applied and environmental microbiology · 2017Article
- Elucidation of the molecular basis for arabinoxylan-debranching activity of a thermostable family GH62 α-l-arabinofuranosidase from Streptomyces thermoviolaceus.Applied and environmental microbiology · 2014Article
- Mapping the polysaccharide degradation potential of Aspergillus niger.BMC genomics · 2012Article
- Fungal enzyme sets for plant polysaccharide degradation.Applied microbiology and biotechnology · 2011Review
- Fungal arabinan and L-arabinose metabolism.Applied microbiology and biotechnology · 2011Review
- Molecular regulation of arabinan and L-arabinose metabolism in Hypocrea jecorina (Trichoderma reesei).Eukaryotic cell · 2009Article
- Aspergillus enzymes involved in degradation of plant cell wall polysaccharides.Microbiology and molecular biology reviews : MMBR · 2001Review
- Endo-xylogalacturonan hydrolase, a novel pectinolytic enzyme.Applied and environmental microbiology · 2000Article
- Arabinanase A from Pseudomonas fluorescens subsp. cellulosa exhibits both an endo- and an exo- mode of action.The Biochemical journal · 1997Article
- Cloning of genes encoding alpha-L-arabinofuranosidase and beta-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae.Applied and environmental microbiology · 1996Article
- Extracellular arabinases in Aspergillus nidulans: the effect of different cre mutations on enzyme levels.Archives of microbiology · 1994Article
- Cloning and characterization of the abfB gene coding for the major alpha-L-arabinofuranosidase (ABF B) of Aspergillus niger.Current genetics · 1993Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Secretion of endo-1,5-alpha-L-arabinase A (ABN A) by an Aspergillus niger xylulose kinase mutant upon mycelium transfer to medium containing L-arabitol was immunochemically followed with time to monitor its induction profile. A cDNA expression library was made from polyA+ RNA isolated from the induced mycelium. This library was immunochemically screened and one ABN A specific clone emerged. The corresponding abnA gene was isolated from an A. niger genomic library. Upon Southern blot analysis, a 3.1-kb HindIII fragment was identified and subcloned to result in plasmid pIM950. By means of co-transformation using the A. niger pyrA gene as selection marker, the gene was introduced in both A. niger and A. nidulans uridine auxotrophic mutants. Prototrophic A. niger and A. nidulans transformants overproduced A. niger ABN A upon growth in medium containing sugar beet pulp as the sole carbon source, thereby establishing the identity and functionality of the cloned gene. The DNA sequence of the complete HindIII fragment was determined and the structure of the abnA gene as well as of its deduced gene product were analysed. Gene abnA contains three introns within its structural region and codes for a protein of 321 amino acids. Signal peptide processing results in a mature protein of 302 amino acids with a deduced molecular mass of 32.5 kDa. A. niger abnA is the first gene encoding an ABN to be isolated and characterized.
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