ArticleApplied microbiology and biotechnology1993
Expression of an alpha-galactosidase gene under control of the homologous inulinase promoter in Kluyveromyces marxianus.
Article in Applied microbiology and biotechnology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 58 citations in OpenAlex.
- A thermostable and highly active fungal GH3 β-glucosidase generated by random and saturation mutagenesis.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Article
- Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture.Scientific reports · 2021Article
- The identification of novel promoters and terminators for protein expression and metabolic engineering applications inMetabolic engineering communications · 2021Article
- Biological Parts forFrontiers in bioengineering and biotechnology · 2019Article
- Genetic and physiological basis for antibody production by Kluyveromyces marxianus.AMB Express · 2018Article
- Improved secretory expression of lignocellulolytic enzymes inBiotechnology for biofuels · 2018Article
- Carbon source dependent promoters in yeasts.Microbial cell factories · 2014Review
- Bacterial acyl-CoA mutase specifically catalyzes coenzyme B12-dependent isomerization of 2-hydroxyisobutyryl-CoA and (S)-3-hydroxybutyryl-CoA.The Journal of biological chemistry · 2012Article
- Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus.Microbial cell factories · 2010Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For expression of the alpha-galactosidase gene from Cyamopsis tetragonoloba in Kluyveromyces marxianus CBS 6556 we have used the promoter of the homologous inulinase-encoding gene (INU1). The INU1 gene has been cloned and sequenced and the coding region shows an identity of 59% with the Saccharomyces cerevisiae invertase gene (SUC2). In the 5'-flanking region of INU1 we found a sequence (TAAATCCGGGG) that perfectly matches to the MIG1 binding consensus sequence (WWWWTSYGGGG) of the S. cerevisiae GAL1, GAL4 and SUC2 genes. Using the K. marxianus INU1 promoter and prepro-signal sequence, we obtained a high alpha-galactosidase production level (153 mg/l) and a secretion efficiency of 99%. Both the production level and the secretion efficiency were significantly reduced when the INU1 pro-peptide was deleted. With either the S. cerevisiae PGK or GAL7 promoter we could obtain only low alpha-galactosidase production levels (2 mg/l).
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