ArticleApplied microbiology and biotechnology1993
Cloning of the Aspergillus niger gene encoding alpha-L-arabinofuranosidase A.
Article in Applied microbiology and biotechnology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Genome analysis of the esca-associated Basidiomycetes Fomitiporia mediterranea, Fomitiporia polymorpha, Inonotus vitis, and Tropicoporus texanus reveals virulence factor repertoires characteristic of white-rot fungi.G3 (Bethesda, Md.) · 2024Article
- Innovations in CAZyme gene diversity and its modification for biorefinery applications.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Rational Design of Mechanism-Based Inhibitors and Activity-Based Probes for the Identification of Retaining α-l-Arabinofuranosidases.Journal of the American Chemical Society · 2020Article
- Insight to Improve α-L-Arabinofuranosidase Productivity inFrontiers in microbiology · 2018Article
- Expression-based clustering of CAZyme-encoding genes of Aspergillus niger.BMC genomics · 2017Article
- Expression ofBiotechnology for biofuels · 2017Article
- Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization.Scientific reports · 2015Article
- Elucidation of the molecular basis for arabinoxylan-debranching activity of a thermostable family GH62 α-l-arabinofuranosidase from Streptomyces thermoviolaceus.Applied and environmental microbiology · 2014Article
- Mapping the polysaccharide degradation potential of Aspergillus niger.BMC genomics · 2012Article
- Fungal enzyme sets for plant polysaccharide degradation.Applied microbiology and biotechnology · 2011Review
- Aspergillus enzymes involved in degradation of plant cell wall polysaccharides.Microbiology and molecular biology reviews : MMBR · 2001Review
- The Pseudomonas cellulosa glycoside hydrolase family 51 arabinofuranosidase exhibits wide substrate specificity.The Biochemical journal · 2001Article
- Purification and characterization of two different alpha-L-rhamnosidases, RhaA and RhaB, from Aspergillus aculeatus.Applied and environmental microbiology · 2001Article
- Purification and substrate specificities of two alpha-L-arabinofuranosidases from Aspergillus awamori IFO 4033.Applied and environmental microbiology · 1998Article
- Purification and characterization of an alpha-L-arabinofuranosidase from Streptomyces lividans 66 and DNA sequence of the gene (abfA).The Biochemical journal · 1994Article
- Cloning and characterization of the abfB gene coding for the major alpha-L-arabinofuranosidase (ABF B) of Aspergillus niger.Current genetics · 1993Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using L-arabitol as an inducer, simple induction conditions were established that resulted in high-level expression of alpha-L-arabinofuranosidase A by an Aspergillus niger D-xylulose kinase mutant strain. These conditions were adapted to construct a cDNA expression library from which an alpha-L-arabinofuranosidase A cDNA clone was isolated using specific antiserum. The corresponding gene encoding alpha-L-arabinofuranosidase A (abfA) was isolated from a genomic library and cloned into a high copy plasmid vector. By co-transformation of uridine auxotrophic mutants lacking orotidine-5-phosphate decarboxylase activity, the afbA gene was introduced both in A. niger and A. nidulans, using the A. niger pyrA gene as selection marker. The identity of the abfA gene was confirmed by overexpression of the gene product by A. niger and A. nidulans transformants, upon growth using sugar beet pulp as the carbon source.
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