ArticlePlanta1993
Cloning and developmental expression of the sucrose-phosphate-synthase gene from spinach.
Article in Planta, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 75 citations in OpenAlex.
- Molecular cloning and expression analysis of sucrose phosphate synthase genes in cassava (Manihot esculenta Crantz).Scientific reports · 2020Article
- De novo and comparative transcriptome analysis of cultivated and wild spinach.Scientific reports · 2015Article
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- Article
- Transgenic cotton over-producing spinach sucrose phosphate synthase showed enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions.Plant molecular biology · 2007Article
- Differential expression of sucrose-phosphate synthase isoenzymes in tobacco reflects their functional specialization during dark-governed starch mobilization in source leaves.Plant physiology · 2005Article
- Light regulation of sucrose-phosphate synthase activity in the freezing-tolerant grass Deschampsia antarctica.Photosynthesis research · 2005Article
- Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses.Plant physiology · 2004Article
- Tissue-specific and developmental pattern of expression of the rice sps1 gene.Plant physiology · 2000Article
- Cloning and expression of a prokaryotic sucrose-phosphate synthase gene from the cyanobacterium Synechocystis sp. PCC 6803.Plant molecular biology · 1999Article
- Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.Journal of bacteriology · 1998Article
- Drought-induced effects on nitrate reductase activity and mRNA and on the coordination of nitrogen and carbon metabolism in maize leavesPlant physiology · 1998Article
- Sucrose-phosphate synthase steady-state mRNA increases in ripening kiwifruit.Plant molecular biology · 1998Article
- Analysis of cDNA clones encoding sucrose-phosphate synthase in relation to sugar interconversions associated with dehydration in the resurrection plant Craterostigma plantagineum Hochst.Plant physiology · 1997Article
- Protein phosphorylation as a mechanism for osmotic-stress activation of sucrose-phosphate synthase in spinach leaves.Plant physiology · 1997Article
- Sucrose biosynthesis in a prokaryotic organism: Presence of two sucrose-phosphate synthases in Anabaena with remarkable differences compared with the plant enzymes.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Cloning and molecular analysis of cDNAs encoding three sucrose phosphate synthase isoforms from a citrus fruit (Citrus unshiu Marc.).Molecular & general genetics : MGG · 1996Article
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- Cloning and expression analysis of sucrose-phosphate synthase from sugar beet (Beta vulgaris L.).Molecular & general genetics : MGG · 1995Article
- Rubisco, rubisco activase and ribulose-5-phosphate kinase gene expression and polypeptide accumulation in a tobacco mutant defective in chloroplast protein synthesis.Photosynthesis research · 1995Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 561-base-pair (bp) polymerase-chain-reaction (PCR) product of sucrose-phosphate synthase (SPS) was amplified using degenerate oligonucleotide primers corresponding to tryptic peptides of SPS (EC 2.4.1.14) from spinach (Spinacia oleracea L). Crucial to the primer specificity and the synthesis of the 561-bp product was the use of primer pools in which the number of degenerate primer species was limited. A full-length cDNA was subsequently obtained by screening a cDNA bacteriophage library with the 561-bp product of SPS and 5' PCR-RACE (Rapid Amplification of cDNA Ends). The 3530-bp cDNA of SPS encoded for a 1056-amino-acid polypeptide of predicted molecular mass of 117 kDa. The deduced amino-acid sequence of spinach SPS showed regions of strong homology with SPS from maize (A.C. Worrell et al., 1991, Plant Cell 3, 1121-1130); amino-acid identity was 54% over the entire protein. Western and Northern analyses of root, petiole and spinach leaf tissue showed that SPS was expressed in an organ-specific manner, being predominantly localized in the leaf. The accumulation of SPS protein and mRNA during leaf development coincided with the early rapid phase of leaf expansion and the apparent transition of the leaf from sink to source status. Levels of SPS mRNA and protein were reduced during the acclimation of leaves to low-irradiance conditions. Transfer of low-irradiance-adapted leaves to higher-irradiance conditions resulted in a gradual increase in SPS protein and mRNA. Diurnal changes in irradiance did not alter SPS protein or transcript levels, indicating that short-term regulation of SPS primarily involves a modulation of enzyme activity.
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