ArticlePlanta1993
Cloning and sequence analysis of a cDNA for barley ferredoxin-dependent glutamate synthase and molecular analysis of photorespiratory mutants deficient in the enzyme.
Article in Planta, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- Client distribution between Chlamydomonas FDX1 and FDX2 in carbon, nitrogen and sulfur assimilation.bioRxiv : the preprint server for biology · 2026Article
- QTL Mapping of Yield, Agronomic, and Nitrogen-Related Traits in Barley (Plants (Basel, Switzerland) · 2024Article
- Expression Analysis of Nitrogen Metabolism-Related Genes Reveals Differences in Adaptation to Low-Nitrogen Stress between Two Different Barley Cultivars at Seedling Stage.International journal of genomics · 2018Article
- Article
- Nitrogen-metabolism related genes in barley - haplotype diversity, linkage mapping and associations with malting and kernel quality parameters.BMC genetics · 2013Article
- Partial sequences of nitrogen metabolism genes in hexaploid wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Glutamate synthase: structural, mechanistic and regulatory properties, and role in the amino acid metabolism.Photosynthesis research · 2005Review
- The evolution of glutamate synthase.Molecular biology reports · 2000Article
- Okadaic Acid Mimics Nitrogen-Stimulated Transcription of the NADH-Glutamate Synthase Gene in Rice Cell Cultures.Plant physiology · 1999Article
- NADH-glutamate synthase in alfalfa root nodules. Genetic regulation and cellular expression.Plant physiology · 1999Article
- Arabidopsis gls mutants and distinct Fd-GOGAT genes. Implications for photorespiration and primary nitrogen assimilation.The Plant cell · 1998Article
- Use of Arabidopsis mutants and genes to study amide amino acid biosynthesis.The Plant cell · 1995Review
- Saccharomyces cerevisiae has a single glutamate synthase gene coding for a plant-like high-molecular-weight polypeptide.Journal of bacteriology · 1995Article
- Existence of two ferredoxin-glutamate synthases in the cyanobacterium Synechocystis sp. PCC 6803. Isolation and insertional inactivation of gltB and gltS genes.Plant molecular biology · 1995Article
- Expression of ferredoxin-dependent glutamate synthase in dark-grown pine seedlings.Plant molecular biology · 1995Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The NH2-terminal sequences of ferredoxin-dependent glutamate synthase (Fd-GOGAT; EC 1.4.7.1) purified from barley (Hordeum vulgare L.) and Chlamydomonas reinhardtii (Dangeard), and of a barley peptide, were determined and the barley sequences were used to design oligonucleotide primers for the polymerase chain reaction. A specific 1.3-kilobase (kb) cDNA fragment specifying the NH2-terminal one-third of the mature barley polypeptide, was amplified, cloned and sequenced. The NH2-terminus of plant Fd-GOGAT is highly conserved and homologous to the NH2-terminus of the heavy subunit of Escherichia coli NADPH-GOGAT. Based on sequence homologies, we tentatively identified the NH2-terminal region of Fd-GOGAT as the glutamine-amidotransferase domain, which is related to the corresponding domain of the purF-type amidotransferases. The Fd-GOGAT cDNA clone, and polyclonal antibodies raised against the barley enzyme, were used to analyse four Fd-GOGAT-deficient photorespiratory mutants. Three mutants (RPr 82/1, RPr 82/9 and RPr 84/82) had no detectable Fd-GOGAT protein in leaves, while the fourth (RPr 84/42) had a small amount of cross-reacting material. Hybridization to Northern blots of total leaf RNA revealed that both RPr 82/9 and RPr 84/82 were indistinguishable from the parental line (Maris Mink), having normal amounts of a 5.7-kb mRNA species. On the other hand, RPr 82/2 and RPr 84/42 each contained two distinct hybridizing RNA species, one of which was larger than 5.7 kb, the other smaller. Using a set of wheat-barley telosomic addition lines we have assigned the Fd-GOGAT structural locus to the short arm of chromosome 2.
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