ArticleProceedings of the National Academy of Sciences of the United States of America1995
Disruption of the gene for hsp30, an alpha-crystallin-related heat shock protein of Neurospora crassa, causes defects in thermotolerance.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 75 citations in OpenAlex.
- Article
- Differential Roles of Three α-Crystallin Domain-Containing sHsps ofInternational journal of molecular sciences · 2022Article
- Duplicate divergence of two bacterial small heat shock proteins reduces the demand for Hsp70 in refolding of substrates.PLoS genetics · 2019Article
- Hsp70 displaces small heat shock proteins from aggregates to initiate protein refolding.The EMBO journal · 2017Article
- Regulated Forms of Cell Death in Fungi.Frontiers in microbiology · 2017Review
- The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species.Microbiology and molecular biology reviews : MMBR · 2016Review
- CZT-1 is a novel transcription factor controlling cell death and natural drug resistance in Neurospora crassa.G3 (Bethesda, Md.) · 2014Article
- Defining the nature of thermal intermediate in 3 state folding proteins: apoflavodoxin, a study case.PLoS computational biology · 2012Article
- Stress-induced cell death is mediated by ceramide synthesis in Neurospora crassa.Eukaryotic cell · 2008Article
- Chaperones in control of protein disaggregation.The EMBO journal · 2008Review
- Characterization of the genomic structures and selective expression profiles of nine class I small heat shock protein genes clustered on two chromosomes in rice (Oryza sativa L.).Plant molecular biology · 2004Article
- Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism.Microbiology and molecular biology reviews : MMBR · 2004Review
- Expression and biochemical characterization of two small heat shock proteins from the thermoacidophilic crenarchaeon Sulfolobus tokodaii strain 7.Protein science : a publication of the Protein Society · 2004Article
- Analysis of interactions between domains of a small heat shock protein, Hsp30 of Neurospora crassa.Cell stress & chaperones · 2002Article
- Alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network.Microbiology and molecular biology reviews : MMBR · 2002Review
- Neurospora clock-controlled gene 9 (ccg-9) encodes trehalose synthase: circadian regulation of stress responses and development.Eukaryotic cell · 2002Article
- Regulation and mechanism of action of the small heat shock protein from the hyperthermophilic archaeon Pyrococcus furiosus.Journal of bacteriology · 2001Article
- Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- A small heat shock protein cooperates with heat shock protein 70 systems to reactivate a heat-denatured protein.Plant physiology · 2000Article
- Small heat shock protein of Methanococcus jannaschii, a hyperthermophile.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
The alpha-crystallin-related heat shock proteins are produced by all eukaryotes, but the role of these proteins in thermoprotection remains unclear. To investigate the function of one of these proteins, we disrupted expression of the single-copy hsp30 gene of Neurospora crassa, using repeat-induced point mutagenesis, and we generated and characterized mutant strains that were deficient in hsp30 synthesis. These strains could grow at high temperature and they acquired thermotolerance from a heat shock. However, the hsp30-defective strains proved to be extremely sensitive to the combined stresses of high temperature and carbohydrate limitation, enforced by the addition of a nonmetabolizable glucose analogue. Under these conditions, their survival was reduced by 90% compared with wild-type cells. This sensitive phenotype was reversed by reintroduction of a functional hsp30 gene into the mutant strains. The mutant cells contained mitochondria from which a 22-kDa protein was readily extracted with detergents, in contrast to its retention by the mitochondria of wild-type cells. Antibodies against hsp30 coimmunoprecipitated a protein also of approximately 22 kDa from wild-type cells. Results of this study suggest that hsp30 may be important for efficient carbohydrate utilization during high temperature stress and that it may interact with other mitochondrial membrane proteins and function as a protein chaperone.
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