ArticlePflugers Archiv : European journal of physiology1995
Characteristics of ecto-ATPase of Xenopus oocytes and the inhibitory actions of suramin on ATP breakdown.
Article in Pflugers Archiv : European journal of physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 85 citations in OpenAlex.
- Septum site placement inMicrobiology (Reading, England) · 2023Article
- Shockwaves induce osteogenic differentiation of human mesenchymal stem cells through ATP release and activation of P2X7 receptors.Stem cells (Dayton, Ohio) · 2013Article
- Erythrocytic adenosine monophosphate as an alternative purine source in Plasmodium falciparum.The Journal of biological chemistry · 2008Article
- OppA, the ecto-ATPase of Mycoplasma hominis induces ATP release and cell death in HeLa cells.BMC microbiology · 2008Article
- Antagonism of ATP responses at P2X receptor subtypes by the pH indicator dye, Phenol red.British journal of pharmacology · 2005Article
- A Mg-dependent ecto-ATPase is increased in the infective stages of Trypanosoma cruzi.Parasitology research · 2004Article
- Release of ATP induced by hypertonic solutions in Xenopus oocytes.The Journal of physiology · 2003Article
- Distinct mechanisms underlying alpha1-adrenoceptor and P2x purinoceptor operated ATP release and contraction in the guinea-pig vas deferens.Neurochemical research · 2001Article
- Actions of a Series of PPADS Analogs at P2XDrug development research · 2001Article
- Coexpression of rat P2X2 and P2X6 subunits in Xenopus oocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Modulatory activity of extracellular H+ and Zn2+ on ATP-responses at rP2X1 and rP2X3 receptors.British journal of pharmacology · 1999Article
- A pyridoxine cyclic phosphate and its 6-azoaryl derivative selectively potentiate and antagonize activation of P2X1 receptors.Journal of medicinal chemistry · 1998Article
- Extracellular nucleotide signaling in the inner ear.Molecular neurobiology · 1998Review
- The P2Y purinoceptor in rat brain microvascular endothelial cells couple to inhibition of adenylate cyclase.British journal of pharmacology · 1996Article
- Selectivity and activity of adenine dinucleotides at recombinant P2X2 and P2Y1 purinoceptors.British journal of pharmacology · 1996Article
- Evidence for a P2-purinoceptor mediating vasoconstriction by UTP, ATP and related nucleotides in the isolated pulmonary vascular bed of the rat.British journal of pharmacology · 1996Article
- P2 purinoceptor-activated inward currents in follicular oocytes of Xenopus laevis.The Journal of physiology · 1996Article
- Action of suramin upon ecto-apyrase activity and synaptic depression of Torpedo electric organ.British journal of pharmacology · 1996Article
- Characteristics of ecto-ATPase of Xenopus oocytes and the inhibitory actions of suramin on ATP breakdown.Pflugers Archiv : European journal of physiology · 1996Article
- P2-purinoceptors mediating spasm of the isolated uterus of the non-pregnant guinea-pig.British journal of pharmacology · 1996Article
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4 authors at 1 institution in 2 countries.
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Abstract
Ecto-ATPase activity of Xenopus oocytes was studied by measuring the production of inorganic phosphate (Pi) from the breakdown of extracellular ATP. Enzyme activity involved Ca2+/Mg(2+)-dependent and Ca2+/Mg(2+)-independent dephosphorylation of ATP. Ca2+/Mg(2+)-dependent ecto-ATPase was active over a limited range of 0.01-1.0 mM ATP, while Ca2+/Mg(2+)-independent ATPase activity was active over a range of 0.1-30 mM ATP. Total enzyme activity was insensitive to changes in buffer pH (pH 7.0-9.0), but increased in a relatively linear manner with: (1) time of reaction (0-90 min), (2) number of cells (1-20 oocytes), and (3) temperature (10-37 degrees C). Ecto-ATPase activity was unaffected by ouabain (100 microM), sodium azide (100 microM), and oligomycin (5 micrograms/ml) (as inhibitors of endo-ATPases) and beta-glycerophosphate (10 mM) and p-nitrophenyl phosphate (10 mM) (as inhibitors of non-specific alkaline phosphatase). Total ecto-ATPase activity was reduced significantly in defolliculated oocytes, suggesting that the enzyme was located mainly on the enveloping follicle cell layer. The range order of preferential substrates was: ATP>GTP, ITP, UTP, CTP, TTP, 2-methylthioATP>ADP, 2-methylthioADP, AMP>>alpha, beta-methylene ATP, beta, gamma-methylene ATP, in the presence of divalent ions (where G is guanosine, I is inosine, U is uridine, C is cytidine and T is ribosylthymine). The P2-purinoceptor antagonist suramin [8-(3-benzamido-4-methylbenzamido)napthalene-1,3,5-trisul phonic acid), 100 microM] significantly inhibited total ecto-ATPase activity; this inhibition was competitive for the Ca2+/Mg(2+)-dependent enzyme.(ABSTRACT TRUNCATED AT 250 WORDS)
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