ArticleMolecular and cellular biology1995
Regulation of urokinase-type plasminogen activator gene transcription by macrophage colony-stimulating factor.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 128 citations in OpenAlex.
- AP1 Transcription Factor in the Regulation of the Urokinase Plasminogen Activation System.Biomolecules · 2026Review
- CSF1R as a Therapeutic Target in Bone Diseases: Obvious but Not so Simple.Current osteoporosis reports · 2022Review
- Therapeutic potential of macrophage colony-stimulating factor in chronic liver disease.Disease models & mechanisms · 2022Article
- Transcriptomic Analysis of Rat Macrophages.Frontiers in immunology · 2020Article
- The evolution of the macrophage-specific enhancer (Fms intronic regulatory element) within the CSF1R locus of vertebrates.Scientific reports · 2017Article
- Transcriptional control ofReproduction (Cambridge, England) · 2017Review
- Enhancer Turnover Is Associated with a Divergent Transcriptional Response to Glucocorticoid in Mouse and Human Macrophages.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Characterisation of a novel Fc conjugate of macrophage colony-stimulating factor.Molecular therapy : the journal of the American Society of Gene Therapy · 2014Article
- Hyperglycemia, a neglected factor during cancer progression.BioMed research international · 2014Review
- Article
- The function of the conserved regulatory element within the second intron of the mammalian Csf1r locus.PloS one · 2013Article
- Article
- Oncogenic ETS proteins mimic activated RAS/MAPK signaling in prostate cells.Genes & development · 2011Article
- Colony-stimulating factor-1 promotes kidney growth and repair via alteration of macrophage responses.The American journal of pathology · 2011Article
- Ras signaling requires dynamic properties of Ets1 for phosphorylation-enhanced binding to coactivator CBP.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Microglia and the urokinase plasminogen activator receptor/uPA system in innate brain inflammation.Glia · 2009Article
- Combinatorial roles of protein kinase A, Ets2, and 3',5'-cyclic-adenosine monophosphate response element-binding protein-binding protein/p300 in the transcriptional control of interferon-tau expression in a trophoblast cell line.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Ets-2 and C/EBP-beta are important mediators of ovine trophoblast Kunitz domain protein-1 gene expression in trophoblast.BMC molecular biology · 2007Article
- Article
- Trophoblast gene expression: transcription factors in the specification of early trophoblast.Reproductive biology and endocrinology : RB&E · 2004Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The mouse urokinase-type plasminogen activator (uPA) gene was used as a model macrophage colony-stimulating factor 1 (CSF-1)-inducible gene to investigate CSF-1 signalling pathways. Nuclear run-on analysis showed that induction of uPA mRNA by CSF-1 and phorbol myristate acetate (PMA) was at the transcriptional level in bone marrow-derived macrophages. CSF-1 and PMA synergized strongly in the induction of uPA mRNA, showing that at least some components of CSF-1 action are mediated independently of protein kinase C. Promoter targets of CSF-1 signalling were investigated with NIH 3T3 cells expressing the human CSF-1 receptor (c-fms). uPA mRNA was induced in these cells by treatment with CSF-1, and a PEA3/AP-1 element at -2.4 kb in the uPA promoter was involved in this response. Ets transcription factors can act through PEA3 sequences, and the involvement of Ets factors in the induction of uPA was confirmed by use of a dominant negative Ets-2 factor. Expression of the DNA binding domain of Ets-2 fused to the lacZ gene product prevented CSF-1-mediated induction of uPA mRNA in NIH 3T3 cells expressing the CSF-1 receptor. Examination of ets-2 mRNA expression in macrophages showed that it was also induced synergistically by CSF-1 and PMA. In the macrophage cell line RAW264, the uPA PEA3/AP-1 element mediated a response to both PMA and cotransfected Ets-2. uPA promoter constructs were induced 60- to 130-fold by Ets-2 expression, and the recombinant Ets-2 DNA binding domain was able to bind to the uPA PEA3/AP-1 element. This work is consistent with a proposed pathway for CSF-1 signalling involving sequential activation of fms, ras, and Ets factors.
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Registered trials
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