ArticleMolecular and cellular biology1995
The upstream region of the FOX3 gene encoding peroxisomal 3-oxoacyl-coenzyme A thiolase in Saccharomyces cerevisiae contains ABF1- and replication protein A-binding sites that participate in its regulation by glucose repression.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 39 citations in OpenAlex.
- Characterization of aromatic residue-controlled protein retention in the endoplasmic reticulum ofThe Journal of biological chemistry · 2017Article
- Article
- Functional characterization of thiolase-encoding genes from Xanthophyllomyces dendrorhous and their effects on carotenoid synthesis.BMC microbiology · 2016Article
- The Sum1/Ndt80 transcriptional switch and commitment to meiosis in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2012Review
- UME6, a novel filament-specific regulator of Candida albicans hyphal extension and virulence.Molecular biology of the cell · 2008Article
- Genome-wide analysis of sterol-lipid storage and trafficking in Saccharomyces cerevisiae.Eukaryotic cell · 2008Article
- Transcriptional control of nonfermentative metabolism in the yeast Saccharomyces cerevisiae.Current genetics · 2003Review
- The Ume6 regulon coordinates metabolic and meiotic gene expression in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Identification of the Sin3-binding site in Ume6 defines a two-step process for conversion of Ume6 from a transcriptional repressor to an activator in yeast.Molecular and cellular biology · 2001Article
- Yeast carbon catabolite repression.Microbiology and molecular biology reviews : MMBR · 1998Review
- Role of UME6 in transcriptional regulation of a DNA repair gene in Saccharomyces cerevisiae.Molecular and cellular biology · 1997Article
- Expression of an ortholog of replication protein A1 (RPA1) is induced by gibberellin in deepwater rice.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Respiration and low cAMP-dependent protein kinase activity are required for high-level expression of the peroxisomal thiolase gene in Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1996Article
- Participation of the yeast activator Abf1 in meiosis-specific expression of the HOP1 gene.Molecular and cellular biology · 1996Article
- A bipartite operator interacts with a heat shock element to mediate early meiotic induction of Saccharomyces cerevisiae HSP82.Molecular and cellular biology · 1995Article
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6 authors at 3 institutions in 2 countries.
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Abstract
Expression of the FOX3 gene, which encodes yeast peroxisomal 3-oxoacyl-coenzyme A thiolase, can be induced by oleate and repressed by glucose. Previously, we have shown that induction was mediated by an oleate response element. Just upstream of this element a negatively acting control region that mediated glucose repression was found. In order to study this negative control region, we carried out DNA-binding assays and analyzed phenotypes of mutations in this region and in the trans-acting factor CAR80, which is identical to UME6. DNA-binding assays showed that two multifunctional yeast proteins, ABF1 and RP-A, interacted with the negative control element independently of the transcriptional activity of the FOX3 gene. ABF1 and RP-A, the latter being identical to BUF, were able to bind to DNA independently of one another but also simultaneously. The phenotypes of mutations in either DNA-binding sites of ABF1, RP-A, or both, which affected the DNA binding of these factors in vitro, indicated that these sites and the proteins that interact with them participate in glucose repression. The involvement of the RP-A site in glucose repression was further supported by our observation that the CAR80 gene product, which is required for repression mediated by the RP-A site, was essential for maintenance of glucose repression. In addition to the RP-A site in the FOX3 promoter, similar sequences were observed in other genes involved in peroxisomal function. RP-A proved to bind to all of these sequences, albeit with various affinities. From these results it is concluded that the ABF1 and RP-A sites are being required in concert to mediate glucose repression of the FOX3 gene. In addition, coordinated regulation of expression of genes involved in peroxisomal function in response to glucose is mediated by proteins associated with the RP-A site, probably RP-A and CAR80.
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