ArticleMolecular and cellular biology1995
The ATF site mediates downregulation of the cyclin A gene during contact inhibition in vascular endothelial cells.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Identification of cyclins A1, E1 and vimentin as downstream targets of heme oxygenase-1 in vascular endothelial growth factor-mediated angiogenesis.Scientific reports · 2016Article
- Runx2, a target gene for activating transcription factor-3 in human breast cancer cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Distamycin A inhibits HMGA1-binding to the P-selectin promoter and attenuates lung and liver inflammation during murine endotoxemia.PloS one · 2010Article
- Homocysteine inhibits endothelial cell growth via DNA hypomethylation of the cyclin A gene.Blood · 2007Article
- The p75NTR-interacting protein SC1 inhibits cell cycle progression by transcriptional repression of cyclin E.The Journal of cell biology · 2004Article
- Promoter specificity and biological activity of tethered AP-1 dimers.Molecular and cellular biology · 2002Article
- Article
- Distinct effects of mitogens and the actin cytoskeleton on CREB and pocket protein phosphorylation control the extent and timing of cyclin A promoter activity.Molecular and cellular biology · 2001Article
- CREB/ATF-dependent repression of cyclin a by human T-cell leukemia virus type 1 Tax protein.Journal of virology · 2001Article
- A family of LIM-only transcriptional coactivators: tissue-specific expression and selective activation of CREB and CREM.Molecular and cellular biology · 2000Article
- Inhibition of cyclin A gene expression in human B cells by an immunosuppressant mizoribine.Clinical and experimental immunology · 2000Article
- The simian virus 40 small-t and large-T antigens jointly regulate cell cycle reentry in human fibroblasts.Journal of virology · 1999Article
- Anchorage-dependent expression of cyclin A in primary cells requires a negative DNA regulatory element and a functional Rb.Oncogene · 1999Article
- Article
- Differential transcriptional regulation of the human thrombin receptor gene by the Sp family of transcription factors in human endothelial cells.The Biochemical journal · 1998Article
- Opposite transcriptional effects of cyclic AMP-responsive elements in confluent or p27KIP-overexpressing cells versus serum-starved or growing cells.Molecular and cellular biology · 1998Article
- TAFII250-dependent transcription of cyclin A is directed by ATF activator proteins.Genes & development · 1997Article
- The role of ATF in regulating the human cytomegalovirus DNA polymerase (UL54) promoter during viral infection.Journal of virology · 1997Article
- A novel simian virus 40 early-region domain mediates transactivation of the cyclin A promoter by small-t antigen and is required for transformation in small-t antigen-dependent assays.Journal of virology · 1996Article
- Cyclin A expression is under negative transcriptional control during the cell cycle.Molecular and cellular biology · 1996Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contact inhibition mediates monolayer formation and withdrawal from the cell cycle in vascular endothelial cells. In studying the cyclins--key regulators of the cell cycle--in bovine aortic endothelial cells (BAEC), we found that levels of cyclin A mRNA decreased in confluent BAEC despite the presence of 10% fetal calf serum. We then transfected into BAEC a series of plasmids containing various lengths of the human cyclin A 5' flanking sequence and the luciferase gene. Plasmids containing 3,200, 516, 406, 266, or 133 bp of the human cyclin A promoter directed high luciferase activity in growing but not confluent BAEC. In contrast, a plasmid containing 23 bp of the cyclin A promoter was associated with a 65-fold reduction in activity in growing BAEC, and the promoter activities of this plasmid were identical in both growing and confluent BAEC. Mutation of the activating transcription factor (ATF) consensus sequence at bp -80 to -73 of the cyclin A promoter decreased its activity, indicating the critical role of the ATF site. We identified by gel mobility shift analysis protein complexes that bound to the ATF site in nuclear extracts from growing but not confluent BAEC and identified (with antibodies) ATF-1 as a binding protein in nuclear extracts from growing cells. Also, ATF-1 mRNA levels decreased in confluent BAEC. Taken together, these data suggest that the ATF site and its cognate binding proteins play an important role in the downregulation of cyclin A gene expression during contact inhibition.
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