ArticleMolecular and cellular biology1995
The Chinese hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sites.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 100 citations in OpenAlex.
- The double life of mammalian DNA replication origins.Genes & development · 2025Review
- The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans.Biology · 2023Review
- Review
- The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing.Nature communications · 2019Article
- 2D gel electrophoresis reveals dynamics of t-loop formation during the cell cycle and t-loop in maintenance regulated by heterochromatin state.The Journal of biological chemistry · 2019Article
- Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeastThe Journal of biological chemistry · 2017Article
- How MCM loading and spreading specify eukaryotic DNA replication initiation sites.F1000Research · 2016Review
- Peaks cloaked in the mist: the landscape of mammalian replication origins.The Journal of cell biology · 2015Review
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- Mapping of Replication Origins in the X Inactivation Center of Vole Microtus levis Reveals Extended Replication Initiation Zone.PloS one · 2015Article
- High-resolution analysis of DNA synthesis start sites and nucleosome architecture at efficient mammalian replication origins.The EMBO journal · 2013Article
- Article
- Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.BMC research notes · 2012Article
- Unraveling cell type-specific and reprogrammable human replication origin signatures associated with G-quadruplex consensus motifs.Nature structural & molecular biology · 2012Article
- New insights into replication origin characteristics in metazoans.Cell cycle (Georgetown, Tex.) · 2012Article
- Genome-scale analysis of metazoan replication origins reveals their organization in specific but flexible sites defined by conserved features.Genome research · 2011Article
- Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.Nucleic acids research · 2011Article
- Article
- A winding road to origin discovery.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Analysis of chromosome III replicators reveals an unusual structure for the ARS318 silencer origin and a conserved WTW sequence within the origin recognition complex binding site.Molecular and cellular biology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previous two-dimensional gel replicon-mapping studies on the amplified dihydrofolate reductase (DHFR) domain in CHOC 400 cells suggested that replication can initiate at any of a large number of sites scattered throughout a 55-kb region lying between two convergently transcribed genes. It could be argued that this unusual distributive initiation mode is unique to amplified chromosomal loci. In this paper, we report the first application of the two-dimensional gel techniques to the analysis of a single-copy locus in mammalian cells. Results obtained with both synchronized and exponentially growing CHO cells suggest that (i) initiation can also occur at any of a large number of sites distributed throughout the intergenic region in the nonamplified DHFR locus, (ii) initiation is confined to the first 2 to 2.5 h of the S period, and (iii) initiation occurs only in a fraction of the DHFR loci in each cell cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.