ArticleMolecular and cellular biology1995
Inhibition of chloroplast DNA recombination and repair by dominant negative mutants of Escherichia coli RecA.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 89 citations in OpenAlex.
- Design and assembly of the 117-kb Phaeodactylum tricornutum chloroplast genome.Plant physiology · 2024Article
- The Evolutionary Constraints on Angiosperm Chloroplast Adaptation.Genome biology and evolution · 2023Article
- Chloroplastic RecA protein fromPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Inheritance through the cytoplasm.Heredity · 2022Review
- Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Plastid Genomes of Flowering Plants: Essential Principles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The caseinolytic protease complex component CLPC1 in Arabidopsis maintains proteome and RNA homeostasis in chloroplasts.BMC plant biology · 2018Article
- The Complete Chloroplast Genome of Catha edulis: A Comparative Analysis of Genome Features with Related Species.International journal of molecular sciences · 2018Article
- High-throughput sequencing of the chloroplast and mitochondrion of Chlamydomonas reinhardtii to generate improved de novo assemblies, analyze expression patterns and transcript speciation, and evaluate diversity among laboratory strains and wild isolates.The Plant journal : for cell and molecular biology · 2018Article
- Dynamic Interplay between Nucleoid Segregation and Genome Integrity in Chlamydomonas Chloroplasts.Plant physiology · 2016Article
- The Complete Plastid Genome of Lagerstroemia fauriei and Loss of rpl2 Intron from Lagerstroemia (Lythraceae).PloS one · 2016Article
- RecA-dependent DNA repair results in increased heteroplasmy of the Arabidopsis mitochondrial genome.Plant physiology · 2012Article
- Expression of complete metabolic pathways in transgenic plants.Biotechnology & genetic engineering reviews · 2012Review
- RecA maintains the integrity of chloroplast DNA molecules in Arabidopsis.Journal of experimental botany · 2010Article
- Creation of a chloroplast microsatellite reporter for detection of replication slippage in Chlamydomonas reinhardtii.Eukaryotic cell · 2008Article
- Chlamydomonas reinhardtii: a convenient model system for the study of DNA repair in photoautotrophic eukaryotes.Current genetics · 2008Review
- Characterization of the 16S-23S internal transcribed spacer among 34 higher plants: suitability for interspecific plastid transformation.Plant cell reports · 2007Article
- Origins and evolution of the recA/RAD51 gene family: evidence for ancient gene duplication and endosymbiotic gene transfer.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Functional specialization of Chlamydomonas reinhardtii cytosolic thioredoxin h1 in the response to alkylation-induced DNA damage.Eukaryotic cell · 2005Article
- Chlamydomonas and Arabidopsis. A dynamic duo.Plant physiology · 2004Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The occurrence of homologous DNA recombination in chloroplasts is well documented, but little is known about the molecular mechanisms involved or their biological significance. The endosymbiotic origin of plastids and the recent finding of an Arabidopsis nuclear gene, encoding a chloroplast-localized protein homologous to Escherichia coli RecA, suggest that the plastid recombination system is related to its eubacterial counterpart. Therefore, we examined whether dominant negative mutants of the E. coli RecA protein can interfere with the activity of their putative homolog in the chloroplast of the unicellular green alga Chlamydomonas reinhardtii. Transformants expressing these mutant RecA proteins showed reduced survival rates when exposed to DNA-damaging agents, deficient repair of chloroplast DNA, and diminished plastid DNA recombination. These results strongly support the existence of a RecA-mediated recombination system in chloroplasts. We also found that the wild-type E. coli RecA protein enhances the frequency of plastid DNA recombination over 15-fold, although it has no effect on DNA repair or cell survival. Thus, chloroplast DNA recombination appears to be limited by the availability of enzymes involved in strand exchange rather than by the level of initiating DNA substrates. Our observations suggest that a primary biological role of the recombination system in plastids is in the repair of their DNA, most likely needed to cope with damage due to photooxidation and other environmental stresses. This hypothesis could explain the evolutionary conservation of DNA recombination in chloroplasts despite the predominantly uniparental inheritance of their genomes.
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